低剂量X射线全身照射对小鼠脾细胞糖皮质激素受体表达的影响

EFFECT OF WHOLE BODY IRRADIATION WITH LOW DOSE X-RANS ON EXPRESSION OF GLUCOCORTICOID RECEPTOR IN MOUSE SPLENOCYTES

采用放射性配体结合法；观察了不同低剂量Ｘ射线全身照射对小鼠脾细胞糖皮质激 素受体（ＧＣＲ）表达的影响及 ７５ｍＧｙ全身照射后 ＧＣＲ表达的时程变化。结果显示， ２５、 ５０、 ７５ｍＧｙ Ｘ射线全身照射后 ８ｈ 小鼠脾细胞 ＧＣＲ表达明显下降； ７５ｍＧｙ Ｘ射线全身照 射后 ４ｈ ＧＣＲ表达开始下降， ８ｈＧＣＲ表达显著低于对照组。低剂量Ｘ射线全身照射可降低小 鼠脾细胞ＧＣＲ的表达，提示 ＧＣＲ表达下调可能在低剂量辐射免疫增强效应中发挥作用。

In this study, the effect of whole body irradiation (WBI) with different low doses of X-rays on the expression of Glucocorticoid receptor (GCR) in mouse splenocytes and the time course of GCR expression after WBI with 75mGy X-rays were observed using the radioactive ligand binding assay. Results showed that the GCR expression in mouse splenocytes decreased significantly 8h after WBI with 25, 50 and 75mGy X-ray irradiation, and in the case of 75mGy, the GCR expression began to decrease 4h after irradiation. Based on the fact that WBI with low doses of X-rays could reduce GCR expression in mouse splenocytes, it suggested that the down-regulated GCR expression may play a role in the enhancement of immune effect caused by low dose radiation.