摘要
目的:研究P-选择素(P-selectin)在ApcMin/+小鼠肠道肿瘤中的作用。方法:采用P-selectin基因缺失的基因工程小鼠和肠道肿瘤模型ApcMin/+小鼠杂交,计数ApcMin/+小鼠与ApcMin/+P-selectin-/-杂交小鼠小肠及大肠肿瘤的数目,并测量其肿瘤体积,研究P-selectin对ApcMin/+小鼠肠道肿瘤的作用。结果:与ApcMin/+小鼠相比,ApcMin/+P-selectin-/-杂交小鼠在9周龄时肠道肿瘤数目与总负荷明显减少。结论:P-selectin缺失能够显著抑制ApcMin/+小鼠肠道肿瘤的生长。
AIM: To investigate the role of P- selectin in intestinal tumorigenesis in Apc^Min/+ mice ( C57BL/ 6J-Apc^Min/+/J). METHODS: Female P- selectin knockout mice (]36. 129S7 -Selp^tmlBay/J, that was P- selectin-/-) were mated with male Apc^Min/+ mice. The offspring was genotyped for Min+/- and P-selectin null mutantions, which were Apc^Min/+ P - selectin-/- mice. The tumor number and gross tumor volume in the small and large intestines of theApc^Min/++ P-selectin-/- mice and Apc^Min/+ mice were determined. REULTS: P -seleetin deficiency in Apc^Min/+ mice resulted in significant decreases in the tumor number and gross tumor volume in the small intestine and total intestine. CONCLU- SION: Deletion of P -selectin significantly inhibits the tumorigenesis in mouse intestines. In addition, the results also sug- gest that P - selectin may be a marker for colon cancer.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2012年第7期1197-1201,共5页
Chinese Journal of Pathophysiology
基金
科技部"973"项目国家重点基础研究发展计划(No.2010CB529702)
国家青年基金资助项目(No.30900764)
关键词
P-选择素
基因工程小鼠
肠道肿瘤
P- selectin
Genetically engineered mice
Intestinal neoplasms