反硝化测定方法的评述

The Comments on Methods for Measuring Denitrification

反硝化作用是氮循环的重要环节,对生态系统的初级生产力、水体质量、大气环境具有重要影响。然而由于大气中N2的背景值很高,使得准确量化反硝化产生的微量N2存在一定的困难;此外,不同研究者在反硝化研究方面缺乏交流,限制了对反硝化测定方法的改进以及对反硝化过程的深入研究。本文综述了目前在陆地和水体生态系统中常用的7种测定反硝化的方法,并对这些方法的优缺点进行了讨论,以期为反硝化测定方法的综合改进提供参考。已有的反硝化测定方法往往只针对于某一种特定的生态系统,具有一定的局限性,未来应加强对已有研究方法的改进,扩大其适用范围,同时加强研制精密度更高的质谱仪和气相色谱仪,以促进反硝化研究的进一步发展。

Denitrification is a key link in N cyc ling, which is important to primary production, water quality and atmosphere environment. Unfortunately, this process is difficult to measure, because the dominant end product of denitrification has very high background concentration in environment, making it difficult to analyze the small increases in N2 concentrations derived from denitrification. Moreover, the lack of communication among different researchers has also impeded denitrification research. In this paper, we review 7 major approaches that have been applied to measure denitrification in terrestrial and aquatic ecosystems and discuss the strengths, weakness, and future prospects for the different methods. The current methods for measuring denitrification are designed to be applicable in specified ecosystem, and future studies should focus on the improvement in denitrification methods and instrumentation to promote the research in denitrificafion.