期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

长雄野生稻(Oryza longistaminata)全基因组Fosmid文库构建 被引量：2

Whole Genome Fosmid Library Construction of Wild Rice(Oryza longistaminata)
下载PDF
导出
摘要 长雄野生稻（0．10ngistaminata）起源于非洲，具有和亚洲栽培稻相同的AA基因组，是栽培稻改良的重要遗传资源。本研究构建了长雄野生稻全基因组fosmid文库，共获得33．6万克隆，文库的平均插入片段大小约为40kb，其中94．7％的克隆插入片段大小在30～50kb之间。挑取其中约11万克隆并保存，可覆盖10倍栽培稻基因组，对任意基因或序列的筛选概率达到了99．99％。将剩余的22万个克隆进行了末端配对测序，显示其有助于长雄野生稻全基因组测序的拼接。所构建的fosmid文库为筛选和克隆长雄野生稻的有利基因提供了研究材料。 Wild rice, Oryza longistaminata, which originated from Africa with the same AA genome as Asian cultivated rice （0. scttiva ）, is an important genetic germplasm for the improvement of cultivated rice. In this study, the constructed O. longistaminata fosmid library contained 336 000 clones. The average size of inserts was about 40 kb, of which 94.7% were distributed from 30 to 50 kb. We selected and preserved 110 000 clones which covered 10-fold cultivated rice genome. The selection probability of specific gene or sequence reached 99.99%. The fosmid library was helpful for sequences assembling after the second-generation sequencing. The remaining 220 000 clones, which underwent end-paired sequencing, revealed its contribution to the assembly of whole genome sequencing of O. longistaminata. This constructed fosmid library will serve as a useful research material to screen and clone favorable genes of O. longistaminata in the future.
作者 李丽娟 蔡晶 张石来 王荔 胡凤益
机构地区 云南农业大学 云南省农业科学院粮食作物研究所 中国科学院昆明动物研究所
出处 《分子植物育种》 CAS CSCD 北大核心 2012年第4期457-461,共5页 Molecular Plant Breeding
基金 国家自然基金项目(U0836605)资助
关键词 长雄野生稻 FOSMID文库 末端测序 Wild rice （Oryaa longistaminata）, Fosmid library, End secquencing
分类号 S511.9 [农业科学—作物学]
  • 相关文献

参考文献11

  • 1Liu L., Lafitte R., and Guan D., 2004, Wild Oryza species as potential sources of drought-adaptive traits, Euphytica, 138(2): 149-161.
  • 2Khush G.S., Bacalangco E., and Ogawa T., 1990, A new gene for resistance to bacterial blight from O. longistaminata, Rice Genet. News1., 7:121-122 Kim C.G., Fujiyama A., and Saitou N., 2003, Construction ofa gorilla fosmid library and its PCR screening system, Genomics, 82(5): 571-574.
  • 3Ammiraju J.S., Yu Y., Luo M., Kudma D., Kim H., Goicoechea J.L., Katayose Y., Matsumoto T., Wu J., Sasaki T., and Wing R.A., 2005, Random sheared fosmid library as a new genomic tool to accelerate complete finishing of dee (Oryza sativa spp. Nipponbare) genome sequence: sequencing of gap-specific fosmid clones uncovers new euehromatic por- tions of the genome, Theor. Appl. Genet., 111(8): 1596-1607.
  • 4Arumuganathan K., and Earle E.D., 1991, Nuclear DNA content of some important plant species, Plant Mol. Biol. Pep., 9(3): 208-218.
  • 5Fitz-Gibbon S., Choi A.J., Miller J.H., Stetter K.O., Simon M.I., Swanson R., and Kim U.J., 1997, A fosmid-based genomic map and identification of 474 genes of the hyperthermophilic archaeon Pyrobaculum aerophilum, Extremophiles, 1(1): 36-51.
  • 6Birren B.W., Tachi-iri Y., Kim U.J., Nguyen M., Shizuya H., Korenberg J.1L, and Simon M.I., 1996, A human chromosome 22 fosmid resource: mapping and analysis of 96 clones, Genomics, 34(1): 97-106.
  • 7Kim U.J., Shizuya H., de Jong P.J., Birren B., and Simon M.I., 1992, Stable propagation of cosmid sized human DNA inserts in an F factor based vector, Nucl. Acids. Res., 20(5): 1083-1085.
  • 8Magrini V., Warren W.C., Wallis J., Goldman W.E., Xu J., Mardis E.R., and McPherson J.D., 2004, Fosmid-based physical mapping of the Histoplasma capsulatum genome, Genome. Res., 14(8): 1603-1609.
  • 9Song W.Y., Wang G.L., Chan L.L., I(im H.S., Pi L.Y., Holsten T., Gardner J., Wang B., Zhai W.X., Zhu L.H., Fauquet C., and Ronald P., 1995, A receptor kinase-likc protein encoded by the rice disease resistance gene, Xa21, Science, 270(5243): 1804-1806.
  • 10Larkin D.M., Everts-van der Wind A., Rebeiz M., Schweitzer P. A., Bachman S., Green C., Wright C.L., Campos E.J., Benson L.D., Edwards J., Liu L., Osoegawa K., Womack J. E., de Jong P.J., and Lewin H.A., 2003, A cattle-human comparative map built with cattle BAC-ends and human genome sequence, Genome. Res., 13(8): 1966-1972.

同被引文献37

  • 1李冰,闫守庆,孙金海.家猪Fosmid基因组文库的构建[J].中国畜牧兽医,2007,34(10):53-55. 被引量：5
  • 2Cohen S N,Chang A C Y,Boyer H W,et al.Construction of biologi-cally functional bacterial plasmids in vitro [J].Proceeding of theNational Academy of Sciences of the United States of America, 1973,70(ll):3240-3244.
  • 3MurryN E,Murry K.Manipulation of restriction targets in phage A toform receptor chromosomes for DNA fragments[ J].Nature, 1974,251(5475):476-481.
  • 4Royal A,Garapin A,Cami B ,et al.The ovalbumin gene region: com-mon features in the organisation of three genes expressed in chickenoviduct under hormonal control [ J] .Nature, 1979,279(5709):125-132.
  • 5MurryA W, Szostak J W.Construction of artificial chromosome inyeast [J].Nature, 1983,305(5931): 189-193.
  • 6ShizuyaH,Birren B,Kim U J,et al.Cloning and stable maintenanceof 300-kilobase-pair fragments of human DNA in Escherichia coliusing an F-factor-based vector[ J].Proceedings of the NationalAcademy of Sciences of the United States of America, 1992,89(18):8794-8797.
  • 7Farr C, Fantes J, Goodfellow P, et al.Functional reintroduction ofhuman telomeres into mammalian cells [ J].Proceedings of theNational Academy of Sciences of the United States of America, 1991,88(16):7006-7010.
  • 8SternbergN.Bacteriophage PI cloning system for the isolation,ampli-fication ,and recovery of DNA fragments as large as 100 kilobase pairs[J].Proceedings of the National Academy of Sciences of the UnitedStates of America, 1990,87(1):103-107.
  • 9Loannou P A, Amemiya C T, Games J, et al.A new bacteriophagePI-derived vector for the propagation of large human DNA fragments[J].Nature Genetics, 1994,6(1):84-89.
  • 10Hosoda F,Nishimura S,Uchida H, et al.An F-factor based cloningsystem for large DNA fragments [ J].Nucleic Acids Research, 1990,18(13):3863-3869.

引证文献2

二级引证文献5

分子植物育种
2012年 第4期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部