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Bcl-2在盐酸异丙肾上腺素诱发大鼠心肌缺血早期的变化 被引量：1

The early change of Bcl-2 In the Iso-induced myocardial ischemia in rats
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摘要 目的研究Bcl-2在盐酸异丙肾上腺素(Isoprenaline hydrochloride,Iso)诱发大鼠心肌缺血早期的变化。方法注射盐酸异丙肾上腺素诱发大鼠心肌缺血模型。采用常规染色,免疫组化染色及RT-PCR方法分析Bcl-2基因和蛋白表达。结果 Bcl-2mRNA表达结果显示:注射Iso后12h,Bcl-2mRNA表达增加;注射24h后,Bcl-2mRNA表达与较12h有所下降,但相对于正常对照组(P<0.05)。Bcl-2蛋白表达随病变程度的加重而降低。结论心肌细胞凋亡是心肌缺血的基础,Bcl-2在早期(12-72h)便参与心肌缺血的全过程。 Objective To investigate the early change in the Iso-induced myocardial ischemia in rats.Methods Rat myocardial ischemia models were induced by isoproterenol（Iso）injection.Conventional staining,immunohistochemical staining and RT-PCR were used to analyze expression of Bcl-2 gene and protein.Results Bcl-2 mRNA arrived peak at 12 h（P0.05）.Bcl-2 protein expression with the severity of disease decreases in 12-72 h.Conclusion Myocardial apoptosis is an important reason of Iso-induced myocardial ischemia,Bcl-2 had a hand in the whole process in the early myocardial ischemia.
作者 李凤 黄冰玉 孙荣江 孙波
机构地区 吉林大学第二医院 东北师范大学人文学院体育教研部 吉林大学药学院
出处 《中国实验诊断学》 2012年第7期1177-1179,共3页 Chinese Journal of Laboratory Diagnosis
关键词 心肌缺血 细胞凋亡 BCL-2 Myocardial Ischemia apoptosis Bcl-2
分类号 R363 [医药卫生—病理学]
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参考文献7

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二级参考文献33

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中国实验诊断学
2012年 第7期

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