寻常型银屑病患者血清MMP-2、IL-18和IL-2的检测及临床意义

Serum concentration of MMP-2,MMP-9,and IL-18 in the patients with Psoriasis Vulgaris and its clinical significance

目的探讨MMP-2、MMP-9和IL-18在寻常型银屑病发生发展过程中的作用。方法以寻常型银屑病患者共73例(包括进行期48例、静止期25例)和健康对照58例样本为试验材料,采用双抗体夹心酶联免疫技术测定血清中MMP-2、MMP-9和IL-18浓度。结果进行期患者血清MMP-2、MMP-9和IL-18浓度均最高,分别为411.57±123.48ng/mL、681.42±297.31ng/mL和421.73±41.30ng/mL,显著高于静止期组和对照组(P<0.05)。治疗后血清中MMP-2、MMP-9和IL-18浓度分别为273.54±91.85ng/mL、431.65±120.81ng/mL和287.34±19.41ng/mL,均显著低于治疗前水平(P<0.05)。结论 MMP-2、MMP-9和IL-18因子在银屑病的发生、发展过程中起着重要作用,观察其浓度变化对探讨其发病机理,预防和指导用药均有重要的临床价值。

Objective We evaluated the biological roles of MMP-2,MMP-9,and IL-18 during the process of Psoriasis Vulgaris.Methods The ELISA method was adopted to detect the serum level of MMP-2,MMP-9,and IL-18 in 73 cases of Psoriasis Vulgaris,including 48 cases in developing psoriasis and 25 remission psoriasis,together with 58 normal persons in control group.Results The highest serum levels of MMP-2,MMP-9,and IL-18 were observed in developing psoriasis cases with 411.57±123.48 ng/mL,681.42±297.31 ng/mL,and 421.73 ± 41.30 ng/mL,respectively,which were significantly higher than that in remission psoriasis and control group.The serum levels of MMP-2,MMP-9,and IL-18 after treatment were 273.54 ± 91.85 ng/mL,431.65 ± 120.81 ng/mL,and 287.34 ± 19.41 ng/mL,which were significantly lower than that before treatment.Conclusion The serum levels of MMP-2,MMP-9,and IL-18 were revealed the significant difference between groups,which would suggest the important biological roles underlying the occurrence and development of Psoriasis Vulgaris disease.