摘要
目的探讨食管癌组织中细胞周期蛋白(CyclinD1、CyclinA、CyclinB1、CyclinE)的表达规律,其与食管癌临床病理参数的关系。方法采用免疫组化技术检测CyclinD1、CyclinA、CyclinB1、CyclinE在58例食管癌组织和45例正常食管黏膜组织中的表达情况。结果 CyclinD1、CyclinA、CyclinB1、CyclinE在正常食管黏膜组织中低表达。随着食管癌分化程度的降低,CyclinD1、CyclinA、CyclinB1、CyclinE表达的阳性率明显升高,高分化组、中分化组与低分化组间有显著差异(P<0.05)。结论可通过免疫组织化学法分析细胞周期的数据,这有利于肿瘤分级,并与预后相关。
Objective To investigate the expression and significance of CyclinD1,CyclinA,CyclinB1 and CyclinE in esophageal carcinomas,and study the correlation between parameters and clinicopathological features.Methods Expression of CyclinD1,CyclinA,Cyclin B1 and CyclinE were analyzed by immunohistochemistry staining in 58 cases of esophageal carcinomas and 45 normal esophageal tissues.Results CyclinD1,CyclinA,Cyclin B1 and CyclinE were low expressed in normal esophageal mucosas.With the decreasing of pathological classification,the expression of CyclinD1、CyclinA、CyclinB1 and CyclinE in esophageal carcinomas increased accordingly.The difference between group highly-differentiated、moderately-differentiated and poorly-differentiated were significant（P0.05）.Conclusion Cell cycle phase can be analyzed by immunohistochemical stain,and it is beneficial for tumor classification and helpful to predict the prognosis.
出处
《中国实验诊断学》
2012年第7期1249-1252,共4页
Chinese Journal of Laboratory Diagnosis
