TERE1(UBIAD1)基因对膀胱癌细胞系T24凋亡的影响

Effects of TERE1(UBIAD1) gene on apoptosis in bladder cancer cell line T24

目的研究外源导入TERE1(UBIAD1)基因对膀胱癌细胞系T24的影响。方法利用脂质体LipofectamineTM2000,将外源TERE1(UBIAD1)基因导入T24细胞。转染TERE1(UBIAD1)基因一定时间后,利用血球计数板计数细胞数目,MTT方法检测细胞活力,流式细胞仪检测细胞周期及凋亡。结果转染基因48 h后,较之对照组,实验组细胞数目减少了80.3%,差异有统计学意义(P=6.6E-07),且随转染时间的延长细胞数目逐渐降低。MTT方法检测到转染TERE1(UBIAD1)基因48 h后实验组细胞活力值为0.4178,较之对照组,细胞活力抑制率达65.6%,差异有统计学意义(P=0.00023)。流式细胞仪检测到转染TERE1(UBIAD1)基因48 h后实验组T24细胞出现凋亡峰,PI和ANNEXIN V-FITC双染实验发现处于凋亡期的细胞占总数的93.73%。结论外源导入TERE1(UBIAD1)基因后膀胱癌T24细胞的数目和活力降低,细胞走向凋亡。

【Objective】 To research the effect of TERE1（UBIAD1） gene which was transfected into bladder cancer cell line T24.【Methods】 TERE1（UBIAD1） gene was transfected into T24 cells through liposome Lipofectamine TM 2000.After a period of time,haemocytemeter was used to count cell number and MTT was used to detect cell vitality.FCM（flow cytometry） was used to detect cell cycle and apoptosis.【Results】 Compared to control group,the cell number of experimental group which was transfected with TERE1（UBIAD1） gene for 48 hours reduced 80.3% and the difference was significant by T-TEST（P =6.6E-07）.The cell number decreased gradually with the transfection time.The vitality value of experimental group which was transfected with TERE1（UBIAD1） gene for 48 hours was 0.4178 which was measured by MTT.The inhibitory rate of cell vitality reached 65.6% compared to normal control group.The difference was significant by T-TEST（P =0.00023）.Apoptosis peak was detected in experimental group which was transfected with TERE1（UBIAD1） gene for 48 hours by FCM.T24 cells of apoptosis in experimental group which was tranfected with TERE1（UBIAD1） gene accounted for 93.73% among the total cells through PI and ANNEXIN V-FITC dye by FCM.【Conclusions】 The number and vitality of T24 cells which were transfected with TERE1（UBIAD1） gene decreased greatly and T24 cells trended to apoptosis.