摘要
转Cry1Ab基因水稻Bt22为一种新型的转基因抗虫水稻,本研究介绍了一种筛选纯合转基因植株Bt22的PCR方法。根据外源序列在Bt22中插入位点的两侧旁邻序列设计引物,建立了转基因抗虫水稻Bt22品系特异的定性PCR检测方法,该方法灵敏度可达0.01 ng,在此基础上组合各引物建立了筛选转基因水稻Bt22纯合体的多重PCR方法,结果表明该方法简单高效,便于操作。
Bt22 is a new type of rice that has been genetically modified to express Cryl Ab protein, we explored a new method based on PCR to screen the homozygous transgenic offspring. The primers were designed on the flanking sequence of the exogenous gene and the specificity and sensitivity of the primers were tested by experiment. Then we established multi-PCR methods to screen homozygous plant from numerous transgenic offspring. Our results showed that this method is simple, efficient and convenient.
出处
《浙江农业学报》
CSCD
北大核心
2012年第4期549-554,共6页
Acta Agriculturae Zhejiangensis
基金
浙江省自然科学基金(Y3090252)
关键词
转基因水稻
特异性定性PCR
多重PCR
纯合性检测
genetically modified rice
event-specific qualitative PCR
multiplex PCR
zygosity testing
