绵羊卵巢运输、保存温度及时间对其卵母细胞孤雌发育能力的影响

Effect of transport and storage temperature and time on parthenogenetically development ability of sheep oocyte

为研究绵羊卵巢不同运输温度、保存温度及时间对其卵母细胞孤雌发育能力的影响,将采集卵巢随机分别置于(10～15),(20～25),(30～35)℃3种不同温度生理盐水中运输到实验室培养,然后优选较好运输温度将采回卵巢随机分为3个试验组,分别保存在4,(14～18),(25～30)℃生理盐水中,保存15～17h后进行成熟培养并孤雌激活。结果表明:(1)在不同运输温度的卵母细胞中,温度为20～25℃时卵母细胞能够进行最好的发育,其成熟率和囊胚率分别为67.44%,35.93%;(2)将绵羊卵巢在14～18℃保存15～17h,对卵母细胞的成熟率(61.81%)、孤雌激活后囊胚的发育率(29.03%)均无显著影响,而将卵巢保存在4℃或25～30℃却显著降低了卵母细胞的成熟率(41.90%,18.40%)与卵裂率(9.09%,13.04%),没有发育到囊胚。从而得出,绵羊卵巢卵母细胞体外培养的长距离运输适宜温度为20～25℃。卵巢在14～18℃保存过夜,不影响卵母细胞的发育能力。

The effect of transport and storage temperature and time on parthenogenetically development ability of sheep oocyte was investigated. Ovary were collected and randomly placed inphysiological saline at 10 -15℃, 20 - 25℃ and 30 -35℃ and transported to the laboratory to culture. They were divided into three groups randomly in optimal transport temperature, respectively kept in 4℃, 14 - 18℃ and 25 -30℃ physiological saline, then oocytes were matured and parthenogenetic activated after 15 - 17 h. The results showed that the optimal transport temperature of ovaries was 20 -25℃, the highest mature rates was 67. 44% and blastocysts rates was 35.93%. When the sheepovaries were kept in 14 - 18℃ for 15 - 17 h, it was found that the corresponding maturation rate（61.81% ） and blastocysts rates （29.03%） of oocytes had no significant difference （P 〉 0. 05 ）. the oocytes mature rates （41.90%, 18.40% ） and the rates of cleavage （9. 09%, 13.04% ） were significantly lower and did not grow to blastocysts when they were kept in 4℃ or 25 - 30℃. It can be concluded that the optimal transport temperature of the sheep o- varies was 20 -25℃, and oocytes of the sheep ovary in vitro had a good growth at 14 -18℃ overnight.