摘要
目的探讨姜黄素(CUR)对大鼠睾丸受X线辐射损伤的保护作用。方法选取雄性SD大鼠60只,体重160~180g,随机分为4组。B组(单纯辐射组)、C组(辐射+溶剂对照组)及D组(辐射+CUR组)大鼠进行剂量为2.0Gy X射线一次性全身照射,对照组(A组)不进行照射。C组大鼠于辐射开始1h后给予腹腔注射二甲基亚砜;D组于辐射开始1h后给予腹腔注射姜黄素溶液。照射1周后将所有大鼠处死。采用TUNEL法检测生殖细胞凋亡;检测睾丸组织中超氧化物歧化酶(SOD)、谷胱甘肽过氧化酶(GSHPx)活性及丙二醛(MDA)含量。结果 B组及C组SOD和GSHPx活性明显降低,MDA含量明显升高,与对照组相比,差异有统计学意义(P<0.01)。而CUR能有效升高SOD和GSHPx活性并降低MDA含量(P<0.01)。B组及C组睾丸脏器系数明显低于正常对照组,差异有统计学意义(P<0.01)。CUR能有效增加睾丸脏器系数(P<0.01)。B组及C组凋亡指数明显高于A组(P<0.01),而CUR可以使凋亡指数显著下降(P<0.01)。结论本实验为CUR作为治疗睾丸辐射损伤的有效物质提供了组织学及生化依据,为临床预防和治疗睾丸辐射损伤提供了理论依据。
Objective To examine the effectiveness of curcumin on X-ray radiation damage of testis tissue in male rats.Methods Male Sprague-Dawley rats(n=60,160~180 g) were divided randomly into 4 groups.Rats in group A served as controls.Rats in group B(radiation group) underwent whole-body X-ray exposure at the dose of 2.0 Gy.Rats in group C and D received DMSO and curcumin respectively 1 hour after 2.0 Gy irradiation.Testicular tissues were prepared 1 week after irradiation.Superoxide dismutase(SOD) activities,glutathione peroxidase(GSHPx) activities,and concentration of malondialdehyde(MDA) were detected.Moreover,the spermatogenic ce1l apoptosis was visualized with TUNEL.Results SOD activities and GSHPx activities in group B and C decreased as compared with those of the controls(P〈0.01).Curcumin treatment significantly increased SOD activities and GSHPx activities(P〈0.05).The MDA concentration in group B and C increased as compared with that of the controls(P〈0.01).Curcumin treatment significantly reduced the MDA concentration(P〈0.01).Irradiation significantly decreased the testis weight(P〈0.01).Curcumin treatment significantly restrained the decrease in testis weight(P〈0.01).Apoptosis indexes in group B and C significantly increased compared with those in the controls(P〈0.01).Curcumin treatment significantly reduced the apoptosis index.Conclusions The results provide a biochemical and histopathological basis to indicate that curcumin acts as a therapeutic agent for the testicular damage induced by X-ray exposure.
出处
《现代泌尿外科杂志》
CAS
2012年第4期365-367,383,共4页
Journal of Modern Urology
关键词
姜黄素
辐射
睾丸
凋亡指数
curcumin
radiation
testis
apoptosis index