模拟CO2气腹对胃癌细胞黏附分子表达的影响

Influence of CO2 pneumoperitoneum pressures on the expression of adhesion molecules of gastriccancer cells

目的研究体外模拟不同压力CO2气腹环境对人胃癌MKN，45细胞表面黏附分子表达的影响。方法体外建立模拟CO2气腹环境，实验组分为3个亚组，气腹压力分别为1．2、1．6和2．0kPa，作用时间均为4h。实验组经气腹处理后第0、24、48、72和96h，对照组普通细胞培养4h，用流式细胞仪检测胃癌MKN-45细胞表面黏附分子CD44v6、细胞间黏附分子1（ICAM-1）和上皮型钙黏蛋白（E-cadherin）表达。结果胃癌MKN-45细胞经1．2、1．6kPaCO2气腹作用后CD44v6和ICAM-1表达呈现先升高后回落，24h时与对照组的差异有统计学意义（P〈0．01），48h时达到高峰，然后逐渐下降，72h时与对照组比较，差异无统计学意义（P〉0．05），96h恢复至对照组水平：E-cadherin表达变化为先降低后回升，处理后即较对照组明显下降（P〈0．01），然后开始回升，48h时与对照组比较，差异无统计学意义（P〉0．05），96h恢复至对照组水平。2．0kPa组CD44v6、ICAM-1和E-cadherin表达变化均更为显著．CD44v6和ICAM-1在处理后即显著高于对照组（P〈0．05）．E-cadherin表达在48h仍然明显低于对照组（P〈0．05）。结论模拟临床常用的CO2气腹压力对胃癌MKN．45细胞表面黏附分子表达可产生一过性影响．但均能在较短时间内恢复。

[Abstract] Objective To study the effect of different CO2 pneumoperitoneum pressures on the expression of adhesion molecules of human gastric cancer cell line MNK-45. Methods MKN-45 cells in the experimental groups were exposed to simulated CO2 environment maintained at different pressures （1.2, 1.6, 2.0 kPa） for 4 hours. Control groups were exposed to room air. At the 0, 24, 48, 72, 96 hours after treatment, CD44v6, ICAM-1 and E-cadherin were detected by flow cytometry method. Results CD44v6 and ICAM-1 expressions showed pattern of firstly elevating, then descending to normal under the pressures of 1.2 kPa and 1.6 kPa. The expressions were different from control group significantly at 24 and 48 hours （P〈0.01）, while the 72 hours expression showed no difference compared with the controls （P〉0.05）. E-cadherin expression decreased significantly right after treatment compared to the control（P〈0.01 ）, but recovered to the level of control at 48 hours（P〉0.05）. In the 2.0 kPa group the expression changes of CD44v6, /CAM-1 and E-cadherin were more remarkable. CD44v6 and ICAM-1 expressions were increased significantly compared to control right after treatment（P〈0.05）. E-cadherin expression was significantly decreased even at 48 hours compared to the controls（P〈0.01 ）. Conclusion In vitro CO2 pneumoperitoneum pressures have transient influence on the adhesion molecules expression of gastric cancer cell MKN-45, then those expressions can recover in a short-time.