基于小鼠表达谱芯片和比较基因组学分析猪生长发育相关候选基因集

Analysis of the candidate gene sets correlated to the growth and development of pigs based on a mouse expression microarray and the comparative genomics

为了在更宽、更深的层面上研究猪生长发育机制,得到以通路为单位的候选基因集以克服传统单基因分析方法的缺点。试验利用模式生物小鼠中的芯片技术,通过基因集富集分析方法(GSEA法)分析基因芯片,确定候选通路。对候选通路成员与猪生长发育相关的数量性状基因座(QTL)数据库进行映射,缩小其作用跨度区域,找出作用的关键基因集。结果表明:得到了56个与猪生长发育相关的候选基因集。

To study the mechanisms of the growth and development of pigs at a wider and deeper level, and to get the candidate gene set using the pathway as a unit to overcome the shortcomings of traditional single gene analysis. The technology of Model Organism （mouse） ChIP -on -chip Microan＇ays was applied, and the gene microarray was analyzed using the gene set enrichment analysis method to identify candidate path- ways. Then, the members of candidate pathways were mapped with the quantitative trait loci （QTL） database correlated to the growth and de- velopment of pigs, which was for decreasing the span area to identify the key gene sets. The results showed that fifty six sets of candidate genes were obtained, which related with the growth and development of pigs.