类黄酮化合物对G-四链体及双链DNA选择性的电喷雾质谱研究

Recognition of Flavonoids Towards to DNA G-quadruplexes and Duplexes by ESI-MS

利用电喷雾质谱(ESI-MS)研究了4种常见的类黄酮化合物芦丁、槲皮素、葛根素和柚皮苷与2种不同形态结构的G-四链体DNA和3种双链DNA的非共价相互作用,比较了这些小分子化合物与不同形态结构DNA结合的强弱及形成复合物的化学计量.结果表明,芦丁和槲皮素对G-四链体DNA具有一定的选择性,同时它们对双链DNA的选择性也较高;而葛根素和柚皮苷对G-四链体DNA仅显示了较低的选择性.

Electrospray ionization mass spectrometry （ESI-MS） was used for the investigation of noncovalent interactions between flavonoids, including rutin, quercetin, naringin, puerarin, and two types of DNA quadruplexes as well as three kinds of DNA duplexes. Percentage bound of each compound towards to different DNA strands were calculated based on the ion abundances of each DNA strand and DNA-ligand complexes obtained in the mass spectra. The results show that rutin and quercetin selectively bound to the G-quadruplexes, meanwhile, they also show strong binding to the duplexes. In contrast, naringin and puerarin show less selectivity towards to the G-quadrulexes.