摘要
基于tdh,trh和tlh 3个基因区分了不同的致病性副溶血性弧菌.采用液相色谱(LC)和气相色谱-质谱联用(GC-MS)技术获得不同的致病性副溶血性弧菌的代谢轮廓,并将其用于区分不同的致病性副溶血性弧菌;同时以肠杆菌基因间重复共有序列聚合酶链反应技术(ERIC-PCR)及DNA重复序列PCR技术(REP-PCR)为对照,采用NTsys2.10e软件计算所得结果的相似系数,并对气相色谱-质谱联用结果进行解析.结果表明,根据所得代谢轮廓可以很好地区分不同的致病性副溶血性弧菌;对气相色谱-质谱联用分析结果解析发现了不同致病性菌株的潜在生物标志物:tdh+,trh-,tlh+菌株3种,tdh-,trh+,tlh+菌株2种,tdh-,trh-,tlh+菌株3种.
Distinguish of different pathogenic vibrio parahaemolyticus are normally based on tdh, trh and tlhgenes. In this research, the metabolic profiles were obtained by liquid chromatography（LC） and gas chromatography/mass spectrometry （ GC-MS）, and they were used for distinguishing different pathogenic vibrio parahaemolytieus. The results were compared with E. eoli gene sequence between the repeat PCR （ERIC- PCR） and DNA repeats-PCR（REP-PCR）. The similarity was calculated by NTsys2. 10e software. The results showed that different pathogenic Vibrio parahaemolyticus could be distinguished by LC and GC/MS. Potential biomarkers of different pathogenic strains were found by GC-MS. Strain of tdh＋ , trh-, tlh＋ have 3 kinds of potential biomarkers, strain of tdh-, trh＋, tlh＋ have 2 kinds of potential biomarkers, strain of tdh-, trh-, tlh＋ have 3 kinds of potential biomarkers.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2012年第8期1686-1691,共6页
Chemical Journal of Chinese Universities
基金
上海市科技兴农重点攻关项目(批准号:沪农科攻字2006第10-5号)
上海市科学技术委员会部分地方院校能力建设项目(批准号:11310501100)
上海市科学技术委员会工程中心建设项目(批准号:11DZ2280300)资助
关键词
副溶血性弧菌
致病性
代谢轮廓
区分
潜在生物标志物
Vibrio parahaemolyticus
Pathogenic
Metabolic profiling
Distinguish
Potential biomarker
