茶树CsH1基因结构及其内含子信息分析

Analysis of genomic structure and introns of CsH1 gene from tea plant

利用cDNA-AFLP方法对低温胁迫下茶树基因表达差异进行了分析,获得1个低温特异表达的cDNA片段。为了获取该片段所代表基因的结构信息,在成功克隆其基因全长cDNA序列(GenBank登录号:EU716314)的基础上,设计引物,从茶树品种‘龙井43’的叶片中克隆获得了该基因全长DNA序列,命名为茶树CsH1基因(JF836005)。基因结构分析表明:CsH1基因包含2个外显子和1个内含子,外显子和内含子的剪接符合GT-AG原则,其中2个外显子的碱基序列长度分别为236 bp和608 bp,内含子为388 bp。经序列分析发现该内含子具有启动子及激素调控元件,可能对CsH1基因的特异性表达有调控作用。茶树CsH1基因的结构及其内含子信息为茶树低温特异基因的表达和抗寒途径分子调控提供了序列信息。

A large-scale screening of cold-induced genes was performed in tea plant（Camellia sinensis（L.）O.Kuntze）by cDNA-amplified fragment length polymorphism（cDNA-AFLP）.Based on a stress-induced H1-histone complete cDNA sequence（GenBank accession No.EU716314）,the complete DNA sequence was cloned from＇Longjing 43＇,named Camellia sinensis H1-histone gene（CsH1）（JF836005）.Gene structure analysis showed that it contained 2 exons and 1 intron,and the splicing principles of its exon and intron were consistent with GT-AG.Two exons were 236 bp and 608 bp,respectively,while the intron was 388 bp.Sequence analysis indicated that the intron had promoter and hormones control components,which may be controlling the specific expression of CsH1 gene.The gene structure and intron of CsH1 gene from tea plant provided sequence information for studies on the expression of cold-induced genes and molecular regulation in cold resistance pathway.