大豆疫霉效应因子PsCRN115诱导烟草抗性的机制分析

The mechanism analysis of induced disease resistance in Nicotiana benthamiana triggered by effector PsCRN115 of Phytophthora sojae

CRN(crinkling and necrosis induced protein)蛋白是卵菌所特有的一类效应因子,在已经测序的疫霉种中,每个基因组中预测都含有上百个CRN基因,关于其功能和分子机制目前研究不多。前期研究发现,大豆疫霉效应因子PsCRN115是大豆疫霉的致病性必需因子且能抑制植物的细胞死亡。为了进一步研究PsCRN115的功能,本研究采用农杆菌渗入法介导的基因瞬时转化技术在烟草上过表达该基因,发现PsCRN115可显著提高烟草对烟草疫霉的抗性。随后采用荧光定量PCR研究了其作用机制,发现PsCRN115可诱导水杨酸(SA)通路防卫反应基因的高表达,但对乙烯(ET)和茉莉酸(JA)通路防卫反应基因的表达影响不显著。表明:大豆疫霉效应因子PsCRN115是致病必需的因子,能被植物所识别,并诱导植物的防卫反应。

CRN（crinkling and necrosis induced protein）effectors,a unique group effectors of oomycetes that contain hundreds of gene in the sequenced genome,were little known about its function and molecular mechanism.We have reported that Phytophthora sojae effector PsCRN115 was necessary for pathogenicity and could suppress plant cell death.Here,we used agro-infiltration mediate transient expression method to overexpress PsCRN115 in Nicotiana benthamiana.The result showed that PsCRN115 could significantly induce plant resistance to the infection of Phytophthora parasitica.Then the quantitative real-time RT-PCR indicated that PsCRN115 could trigger up-regulation of defense response genes in salicylic acid（SA）signaling pathway,but it was not related to ethylene（ET）and jasmonic acid（JA）signaling pathway.Those results indicated that PsCRN115 effector could be used as an elicitor to induce plant disease resistance.