TDZ和6-BA对大豆子叶节再生体系中丛生芽诱导的效应

Effects of TDZ and 6-BA on inducing multiple shoots in soybean cotyledonary node regeneration system

以栽培大豆品种‘Jackson’为试材,用萌发5 d的子叶节作外植体,接种于不同激素配比的萌发培养基和诱导培养基中,分析和比较TDZ(噻二唑苯基脲)和6-BA(6-苄基腺嘌呤)单独和复配使用时对大豆子叶节再生体系中丛生芽的诱导效应,并对其优化组合进行了筛选。结果表明:在种子萌发培养基中,0.5 mg·L-16-BA或TDZ单独使用时,前者能使丛生芽诱导率达到75.56%,诱导效应好于后者,但每个子叶节出芽数低于后者。在诱导培养基中,0.5 mg·L-16-BA和1.0 mg·L-1TDZ复配使用时,丛生芽诱导率最高且每个子叶节出芽数最多,分别为84.75%和3.04。

The soybean cultivar ＂Jackson＂ was used as the experimental material in this study.The soybean cotyledonary nodes from germinated seeds for 5 d were used as the explant and inoculated onto the different culture media for seed germination and multiple shoot induction.The different effects of TDZ（thidiazuron）,6-BA（6-benzyladenine）and their optimized combination on inducing multiple shoots in soybean cotyledonary node regeneration system were investigated.The results showed that,when 0.5 mg·L-1 6-BA or TDZ was separately added to the seed germination medium,the regeneration rate of multiple shoots for the former could reach 75.56%,which was higher than the latter,but the budding number of each cotyledonary node showed the reverse.When 0.5 mg·L-1 6-BA and 1.0 mg·L-1 TDZ were simultaneously added to the induction medium,the regeneration rate of multiple shoots and the budding number of each cotyledonary node could reach 84.75% and 3.04 at the maximum level,respectively.