核黄素和接种番茄黄化曲叶病毒对番茄几丁质酶和β-1,3-葡聚糖酶活性的影响

Effects of riboflavin and TYLCV inoculation on the activities of chitinase and β-1,3-glucanase in tomato

以番茄品种‘1479’为材料,研究喷施核黄素(riboflavin)和接种番茄黄化曲叶病毒(TYLCV)对番茄叶片几丁质酶和β-1,3-葡聚糖酶活性的影响。结果表明:喷施处理后96 h内,2.0 mmol·L-1核黄素能显著提高番茄叶片的几丁质酶和β-1,3-葡聚糖酶活性;接种TYLCV后,核黄素进一步诱导番茄叶片的酶活性显著升高,接种处理的几丁质酶和β-1,3-葡聚糖酶活性均高于未接种处理。表明核黄素诱导病程相关蛋白酶活性增强与番茄对TYLCV的诱导抗性密切相关。

With tomato cultivar＇1479＇as materials,effects of spraying riboflavin and inoculating tomato yellow leaf curl virus（TYLCV）on the activities of chitinase and β-1,3-glucanase in tomato leaves were investigated.The results showed that the activities of chitinase and β-1,3-glucanase increased significantly in tomato leaves within 96 h after spraying 2.0 mmol·L-1 riboflavin.After inoculating TYLCV,the activities of enzymes in tomato leaves further significantly increased after inducing by the riboflavin.The activities of chitinase and β-1,3-glucanase in plants inoculated with TYLCV were higher than those of un-inoculated plants.The above results indicated that the systematically-enhanced activities of the enzymes by riboflavin were closely correlated to the induced resistance of tomato to TYLCV,which might belong to the metabolism of systemic acquired resistance.