HPLC测定贵州不同产地的七叶一枝花中9种甾体皂苷的含量

Determination of nine steroidal saponins in Paris polyphylla from different areas of Guizhou province by HPLC

目的:通过比较贵州不同产地七叶一枝花中的主要活性成分甾体皂苷的种类和含量,为进一步探讨贵州地区作为七叶一枝花药材种植基地提供参考依据。方法:采用HPLC-UV测定来源于贵州省8个不同产地的七叶一枝花中9种甾体皂苷重楼皂苷Ⅶ,偏诺皂苷-3-O-α-L-rha(1→2)[α-L-rha(1→4)]-β-D-glc(PGRR),重楼皂苷H,重楼皂苷Ⅵ,重楼皂苷Ⅱ,薯蓣皂苷,纤细薯蓣皂苷,重楼皂苷Ⅰ及重楼皂苷Ⅴ的含量。结果:重楼皂苷Ⅶ,PGRR,重楼皂苷H,重楼皂苷Ⅵ及纤细薯蓣皂苷5个甾体皂苷均可检测到,这可为增加鉴别重楼药材提供依据;独山产地的七叶一枝花中甾体皂苷含量最高(9.62%),其次为铜仁产地(6.39%),含量最低的为遵义产地的药材(0.99%)。结论:贵州不同产地的皂苷种类和含量差别很大,适宜作为种植基地的为独山地区。

Objective： To compare steroidal saponins-main active ingredients of Paris polyphylla from different areas in Guizhou province,in order to provided basis for further proving Guizhou province to be the planting base of P.polyphylla.Method： The contents of nine steroidal saponins,namely paris-Ⅶ,（25R）-5-en-spirost-3β,17α-diol-3-O-α-L-rhamnopyranosyl-（1→2）[α-L-rhamnopyranosyl（1→4）]-β-D-glycopyanoside（PGRR）,paris-H,paris-Ⅵ,paris-Ⅱ,paris-Ⅲ,gracillin,paris-Ⅰand paris-Ⅴof P.polyphylla from eight areas of Guizhou province were determined by HPLC-UV.Result： Five steroidal saponins-paris-Ⅶ,PGRR,paris-H,paris-Ⅵ and gracillin were detected in all the drugs,which provided basis for distinguishing P.polyphylla.P.polyphylla from Dushan showed the highest content of steroidal saponins（9.62%）,followed by Tongren（6.39%）,and the lowest was Zunyi（0.99%）.Conclusion： Saponins from different areas of Guizhou province show significant difference in variety and content,therefore Dushan is suitable for planting P.polyphylla.