TCRγδ^+CD^(3+)CD^(4-)CD^(8-)双阴性T细胞在3型鼠肝炎病毒诱导的小鼠慢性病毒性肝炎发病中的作用

Changes of TCRγδ^+CD3^+CD4^-CD8^- double negative T cells in mice with MHV-3-induced viral hepatitis

目的本研究拟探讨在MHV-3诱导的慢性病毒性肝炎模型小鼠外周血和脾脏TCRγδ+DNT细胞在T淋巴细胞中比例变化以及其主要表面分子标记的变化。方法取60只C3H/Hej小鼠,腹腔注射10pfu MHV-3;应用流式细胞仪三色或四色荧光分析法分别检测小鼠外周血和脾脏TCRγδ+DNT细胞在T淋巴细胞中的比例及其CD25、CD28、CD30和CD44表达的变化。结果 MHV-3诱导的慢性病毒性肝炎小鼠各时间点外周血和脾脏TCRγδ+DNT在T淋巴细胞中的比例较对照组显著升高(P<0.05);在感染后10天达到最高峰:外周血和脾脏TCRγδ+DNT细胞占T淋巴细胞的比例分别为4.8±1.5%和4.5±1.3%;模型小鼠外周血和脾脏TCRγδ+DNT细胞的表型均为TCRγδ+CD3+CD4-CD8-CD25-CD28-CD30-CD44+。结论感染MHV-3后C3H/Hej小鼠体内TCRγδ+DNT细胞的数量增加,提示TCRγδ+DNT细胞参与了MHV-3诱导的小鼠慢性病毒性肝炎的发生和发展过程。

Objective The arm of this study was to investigate the changes of TCRγδ＋CD3＋CD4-CD8（TCRγδ＋DNT） cells in C3H/Hej mice with murine hepatitis virus type 3（MHV-3）-induced viral hepatitis.Methods MHV-3-induced viral hepatitis was established.At day 0,3,7,10,15 and 20 after MHV-3 infection,the TCRγδ＋DNT cells in peripheral blood and spleen were counted by fluorescent antibodies staining and detected by flow cytometry for its proportion to CD3＋T cells and the expression of CD25,CD28,CD30 and CD44 of the cells were identified.Results After MHV-3 infection,the proportion of TCRγδ＋DNT to CD3＋T cells in peripheral blood and spleen were markedly increased（P〈0.05） and it peaked at day 10（4.8±1.5% and 4.5±1.3% in peripheral blood and spleen,respectively）;The analysis of cell surface marker demonstrated that both of the phenotypes of TCRγδ＋DNTs in peripheral blood and spleen were TCRγδ＋CD3＋CD4-CD8-CD25-CD28-CD30-CD44＋.Conclusions The increase of TCRγδ＋DNT in peripheral blood and spleen may play an important role in the pathological process of MHV-3-induced viral hepatitis.