麻疹病毒沪191对HeLa肿瘤细胞抑制作用的体内外实验研究

Antitumor Effect of Measle Virus Strain Hu 191 on HeLa Cell Lines in vitro & in vivo Tests

研究麻疹病毒减毒疫苗沪191株(MV沪191)在组织培养中和裸鼠体内对HeLa肿瘤细胞的抑制作用。用空斑实验测定MV沪191感染HeLa细胞后细胞裂解液中病毒量;用MTT试验测定MV沪191感染对细胞活性的影响;用流式细胞仪分析测定MV沪191感染引起的细胞凋亡和对细胞周期的影响;HeLa肿瘤细胞背部皮下接种BALB/C裸鼠引起的肿瘤,评估MV沪191体内抑瘤作用。MV沪191感染HeLa细胞后可引起广泛的CPE,感染的HeLa细胞与对照组相比细胞活性明显降低。MV沪191感染HeLa细胞后随着时间延长,G1/G0细胞率明显增多,S期率明显减少,细胞凋亡率明显增加(P<0.01)。给药第60天时瘤内治疗组、静脉治疗组和对照组肿瘤体积平均分别为15.5、64.6、156.4 mm3。瘤内治疗组与对照组相比有显著差异(P<0.01);静脉治疗组与对照组相比有明显差异(P<0.05)。MV沪191减毒株在组织培养中和裸鼠体内对HeLa肿瘤有明显的杀伤作用。

The therapeutic effect of measles virus Hul91 （MV191） on HeLa cell cultures and on nude mice bearing HeLa tumors was studied. Viral quantity in cells were assayed using plaque experiments. The cytotoxie effect of MV191 on HeLa cell in vitro was detected by MTT assay. The changes of cell cycle and apoptosis rate of HeLa cells infected by MV191 were assayed by flow cytometcr. Subcutaneous HeLa tumor model were established in the hind flanks of nude mice and investigated antitumor activity of MV191 against HcLa xenografts in experiments in vivo. The results showed that widespread cytopathic effects （CPE） were observed on the monolaycrs of HeLa cell infected ; The viability of HeLa cells infected have reduced obviously. The infection by MV 191 could cause G1 cells retardation in HeLa cells. The apoptosis rate of tumor cells increased obviously as compared with the untreated control cells. There was a significant suppression of tumor growth in MV191 treated intratumorally animals （P 〈 0.01 ） as compared with controls. Therefore, MV191 had potent antitumor activity against HeLa cell lines and its xcnografts.