藤茶植株叶果与组培物中二氢杨梅素含量的比较研究

Compare of Contents of Dihydromyricetin in Leaf,Fruit and Tissue Culture Objects of Ampelopsis grossedentata

为比较藤茶经组织培养后不同组织中二氢杨梅素的含量,采用高效液相色谱法(HPLC)测定藤茶叶片、果实、悬浮培养液、嫩叶、愈伤组织、老叶中二氢杨梅素的含量。结果表明:嫩叶、叶片、老叶、果实、愈伤组织、悬浮培养液中二氢杨梅素的含量分别为:25.528%、13.709%、13.337%、6.255%、11.616%、0.829%。愈伤组织中二氢杨梅素含量与叶片接近,悬浮液中含量最低,可以尝试运用愈伤组织培养法生产二氢杨梅素。

In order to compare the contents of dihydromyricetin in leaf, fruit and tissue culture objects of Ampelopsis grossedentata, the content of dihydromyricetin in immaturate leaf, mature leaf, post-mature leaf, fruit, suspension cultures, callus of Ampelopsis grossedentata were determined by HPLC. The results showed that the contents of dihydromyricetin in immaturate leaf, mature leaf, post-mature leaf, fruit, suspension culture solution, callus of Ampelopsis grossedentata were 25.528%, 13.709%, 13.337%, 6.255%, 0.829% and 11.616%, respectively. The content of dihydromyricetin in mature leaf was similar to that in callus, and the content of dihydromyricetin in suspension culture solution was the lowest. It is a try to produce dihydromyricetin through callus culture method in future.