中国部分地区羊泰勒虫病的流行病学调查及分类鉴定

Epidemiological Survey and Identification of Theileria Parasite Infection for Small Ruminants in Some Parts of China

【目的】调查近年来中国羊泰勒虫病的流行现状,并对其分离株进行分子分类学鉴定。【方法】对分离自2005—2011年间的羊血液基因组以及蜱基因组,依次用吕氏泰勒虫、尤氏泰勒虫和绵羊泰勒虫特异引物进行PCR扩增。对阳性样品进行18S rRNA基因的特异扩增和测序分析,并建立系统发育树。【结果】对所有羊血液基因组和蜱基因组的PCR扩增表明,在中国所调查的4省9县市,羊泰勒虫病的流行存在明显差异。甘肃省羊泰勒虫病的发病率和感染率较高,主要呈吕氏泰勒虫和尤氏泰勒虫混合感染,现场调查危害比较严重。新疆喀什仅存在绵羊泰勒虫,调查没有见到临床发病病例。在湖北样品中检测到吕氏泰勒虫,但未发现临床上发病病例。在云南样品中没有检测到羊泰勒虫及其发病病例。【结论】羊泰勒虫病在中国上述区域呈现不同程度的发病率和感染率,该研究为这些区域羊泰勒虫病的综合防治提供了参考依据。

[Objective]The objective of this experiment is to investigate the current epidemiological status ofovine and caprine theileriosis and identified its pathogens in China. [Method] The genomic DNA of the blood samples of sheep and goats and ticks were amplified using specific primers to T. uilenbergi, T. luwenshuni and T. ovis, respectively. The 18S rRNA gene sequences from the positive samples were obtained and used for phylogenetical analysis. [Result] On the basis of the PCR results, wide spreading of the theileriosis and significant difference in terms of pathogen species and prevalence in the investigated sites were observed. Co-infection of T. uilenbergi and T. luwenshuni was found only in Gansu Provinces. T. ovis infection was found in Kashi, Xinjiang, but T. luwenshuni infection was detected in Hubei Province but no clinical cases were found. None Theileria infection in Yunnan Province was found. [ Conclusion] The prevalence and infection situation of ovine and caprine theileriosis varied markedly in the studied sites. The data will be useful for guiding the comprehensive prevention and control of ovine and caprine theileriosis in these regions.