郁怒诱发经前期综合征肝气郁证猕猴模型血清单胺类神经递质含量分析

Analysis on content of serum monoamine neurotransmitters in macaques with anger-in-induced premenstrual syndrome and liver-qi depression syndrome

目的:观察猕猴血清中单胺类神经递质含量的变化,探讨血清中单胺类神经递质在郁怒诱发经前期综合征(premenstrual syndrome,PMS)肝气郁证猕猴模型中的作用。方法:以雌性实验猕猴为对象,分为正常组、郁怒情绪反应组、PMS肝气郁证模型组和治疗组。应用社会等级压力应激法诱导猕猴出现"怒"的情绪反应,然后筛选出郁怒情绪反应猕猴进行择时挤压造模,制备PMS肝气郁证猕猴模型。造模成功后,采用雌性实验猕猴情绪评价量表进行行为学评价,并于卵泡期或黄体中晚期采集猕猴血清,分析血清去甲肾上腺素、多巴胺、5-羟色胺含量。结果:与正常对照组相比,PMS肝气郁证模型组和郁怒情绪反应组猕猴的量表抑郁情绪积分显著高于对照组(P<0.01,P<0.05),且造模组显著高于情绪反应组(P<0.05),用药后恢复正常;与正常对照组相比,PMS肝气郁证模型组和郁怒情绪反应组猕猴血清单胺类神经递质含量显著升高(P<0.05,P<0.01),肝气郁证模型组也显著高于情绪反应组(P<0.05),用药后恢复正常。结论:郁怒情绪极易诱发PMS肝气郁证,其机制与单胺类神经递质改变有关。

OBJECTIVE： To observe the changes in content of monoamine neurotransmitters in the serum of rhesus macaques, and explore the role of serum monoamine neurotransmitters in premenstrual syndrome （PMS） and liver-qi depression induced by anger-in emotion. METHODS： Social level pressure was applied on 24 female macaques to induce the angry emotional reaction, and then nine of the low-status macaques with anger-in emotional reaction were screened out and were divided into anger-in emotion group, PMS and liver-qi depression group （model group） and Jingqianshu Granule group. Macaques in the last two groups were suffered extruding in a pack cage for inducing PMS liver-qi depression. After 5 d of extruding, experimental animals were evaluated according to the emotional evaluation scale, meanwhile, macaque serum of follicular phase and middle-late luteal phase was collected to analyze the content of serum norepinephrine, dopamine, and 5-hydroxytryptamine. RESULTS： Compared with the normal control group, the scores of depression of the model group and the anger-in emotion group evaluated with emotional evaluation scale were significantly increased （P〈0. 01, P〈0.05） ; while the score of the model group was significantly higher than that of the anger-in emotion group （P〈0.05）, and it returned to normal after Jingqianshu Granule treatment. As compared to the normal control group, serum monoamine neurotransmitter levels of the model group and the anger-in emotion group were increased （P〈0.05, P〈0.01）, and the serum monoamine neurotransmitter levels of the model group were significantly higher than those of the anger-in emotion group （P〈0. 05）, while there was no significant difference when compared with the normal control group after the treatment. CONCLUSION： Anger-in emotion can induce liver-qi depression syndrome which is related to the changes in monoamine neurotransmitters.