成纤维细胞生长因子7、10及其受体2Ⅲb在小鼠肾脏发育中的表达

Expression of FGF7,FGF10 and FGFR2Ⅲb in the process of development of mouse kidney

目的观察成纤维细胞生长因子7、10(FGF7、FGF10)及其受体2Ⅲb(FGFR2Ⅲb)在不同发育阶段的小鼠肾脏的表达变化情况,探讨其与肾脏发育的关系。方法选取胚龄E12、14、16、18d胎鼠和生后N1、3、7、14、21和42d仔鼠肾脏,应用免疫组织化学技术对小鼠肾组织中FGF7、FGF10和FGFR2Ⅲb表达进行定位观察;应用体视学方法及Western blotting法对小鼠肾组织中FGF7、FGF10和FGFR2Ⅲb表达进行定性分析和定量检测。结果免疫组织化学法显示,E12d时,FGF7、FGF10和FGFR2Ⅲb开始在输尿管芽微弱表达;随着肾脏发育成熟,三者主要表达于远端小管和集合管。FGF10在近曲小管表达,近直小管无表达;FGF7和FGFR2Ⅲb在近端小管无表达。生后肾组织及发育各阶段肾小体未见FGF7、FGF10和FGFR2Ⅲb的表达。体视学和Western blotting结果显示,从E14d至N7d,三者在肾脏的表达量明显增加,N7d至N42d表达量逐渐减少。结论 FGF7、FGF10和FGFR2Ⅲb在肾脏发生和发育过程中可能起着重要作用。FGF10主要作用于近曲小管、远端小管和集合管,而FGF7和FGFR2Ⅲb主要作用于远端小管和集合管。

Objective To observe the expressions offibroblast growth factor 7 and 10 （FGF7, FGF10） and fibroblast growth factor receptor 2 Ⅲ b （FGFR2 Ⅲ b） in kidney tissue of mice during different development stages, and investigate the relationship between FGF7, FGF10, FGFR2Ⅲb and renal development. Methods The kidneys of mice of fetal age 12, 14, 16 and 18d and neonatal age of 1, 3, 7, 14, 21 and 42d were selected. The expressions of FGFT, FGF 10 and FGFR2 Ⅲ b were determined with immunohistochemistry and Western blotting, and qualitatively and quantitatively analyzed with stereological method. Results Immunohistochemical staining showed that FGF7, FGF10 and FGFR2Ⅲb were weakly expressed in the ureteric buds at the fetal age of 12d （E12d）, and then, along with the renal development, they expressed mainly in distal tubules and collecting ducts. FGF 10 was expressed in the proximal convoluted tubule, but not in the proximal straight tubule, while no expression of FGF7 or FGFR2 Ⅲ b was found in the proximal tubule. No expressions of FGF7, FGF 10 and FGFR2 Ⅲb were found in the metanephrogenic tissue and corpuscular renis in each development stage. Western blotting and stereological analysis revealed that the expressions of FGF7, FGF 10 and FGFR2 Ⅲb increased obviously from E14 to N7, and then decreased gradually from N7 to N42. Conclusions FGFT, FGF10 and FGFR2 Ⅲb might play an important role in the renal development. FGF10 plays its role mainly in proximal convoluted tubule, distal tubule and collecting duct, while FGF7 and FGFR2 Ⅲ b play their roles dominantly in distal tubule and collecting duct.