五子衍宗复方总多糖对慢性酒精中毒大鼠肝脏的保护作用

Protective effect of total polysaccharides in Wuzi-Yanzong pill on chronic alcohol-induced injury in Rat

目的在前期研究工作的基础上,对具有肝脏保护作用的中药五子衍宗复方(WZ)进行进一步的分离,考察WZ复方提取物中总多糖(TPC)对慢性酒精中毒大鼠肝脏的保护作用并探讨其可能的作用机制。方法本实验采用改良的Lieber-DeCarli液体酒精饲料喂养致慢性酒精性肝损伤大鼠模型,应用TPC治疗,观察血清生化指标和病理学改变,考察对肝脏氧化应激、脂质过氧化指标和细胞因子TNF-α水平及TNF-αmRNA表达影响。结果 TPC能够显著降低升高的肝脏指数和血清ALT、AST水平,减轻肝脏组织病理学改变;减轻肝脏脂质过氧化,显著恢复GSH含量和SOD活性,从蛋白和基因水平抑制长期酒精诱导所致的细胞因子TNF-α过度表达。结论 TPC对慢性酒精大鼠肝脏具有显著的保护作用,其肝脏保护作用可能与其抗氧化应激,抑制炎性细胞因子TNF-α表达和含量有关。

Objective To investigate the protect effect and mechanism of total polysaccharides （TPC） isolated from 50% eth- anol extract of Wuzi-Yanzong pill （WZ） against chronic alcohol-induced injury in Rat . Methods The effect of TPC on chronic etha- nol hepatotoxicity was investigated in modified Lieber-DeCarli rat model. Hepatocellular damage indicator （ Serum ALT, AST） and his- topathological examination of liver in rat were evaluated. MDA content, SOD activity and GSH content in liver were measured. TNF-α content and TNF-α mRNA expression were determined. Results TPC protected against chronic ethanol-induced hepatotoxicity in rat, as evidenced by decreased plasma transaminases activities and signally improve the liver pathological changes induced by alcohol. TPC significantly inhibited the increase of MDA content, improved SOD activity and prevented GSH depletion. TPC significantly alleviated hepatic TNF-α production and decreased the expression of TNF-α mRNA. Conclusion TPC possessed potent bepatoprotective activi- ties in chronic alcohol-induced injury rat model. The suppressive effect of TPC on chronic alcohol induced liver injury might be attrihu- ted to the antioxidant action and suppress TNF-α expression at protein and zene level.