一种高效硝化菌联合体的分离

Isolation of a High Efficient Nitrobacteria Union

通过最大或然数液体分离法和固体平板分离法从武汉市西湖的湖泊底泥中分离得到了一种以氨盐为唯一能源,具有硝化作用的硝化菌联合体———A2-6-3。经16S rDNA菌种鉴定,此硝化菌联合体包含的菌种归属于硝化杆菌属、硝化螺旋菌属、红假单胞菌属,其中以硝化螺旋菌属为主。对硝化菌联合体A2-6-3进行硝化速率和传代稳定性研究,发现其将氨氮转化为硝态氮的平均硝化速率为4.02 mg/(L.d),最大硝化速率达到13.99 mg/(L.d)。硝化菌联合体A2-6-3的菌群组成和硝化性能传代后保持稳定。

A nitrobacteria union called A2-6-3 which uses ammonia nitrogen as the only energy and has efficient nitrification was isolated from substrate sludge of Xihu Lake in Wuhan by the separation of most probable number and solid plate. 16S rDNA identification of A2-6-3 showed that the union included Nitrobacter, Nitrospira and Rhodopseudonomas, with Nitrospira as the main bacteria. Study on nitrification rates and stability of subculture of A2-6-3 found that it can transform ammonia nitrogen into nitrate nitrogen, with average nitrification rates as 4.02 mg/（L d） and the maximum nitrification rates as 13.99 mg/（L d）. Besides that the composition and nitrification rates of A2-6-3 keep stability after subculture.