DA-Lewis大鼠肝移植急性排斥模型免疫状态的观察

Observation of immune status in acute rejection model of DA-Lew rat liver transplantation

目的通过建立DA-Lewis大鼠肝移植急性排斥模型,研究急性排斥反应的发生发展。方法实验分为两组:同基因(Lewis-Lewis)组24例;异基因(DA-Lewis)组24例。分别于术后第3、5、7、10天随机取3只受体鼠处死取标本并对大鼠肝脏进行检测,观察其病理变化,并对天冬氨酸转氨酶、总胆红素及细胞因子水平变化进行检测。结果异基因组大鼠在术后各个时间点观察的一般表现、肝功能及病理改变、细胞因子水平的变化,都与同基因组存在显著的差别,其排斥反应观察的最佳时间是第7天。结论选择DA-Lewis大鼠建立肝移植急性排斥模型,第7天出现重度排斥反应,可作为反应排斥程度检测指标的时间点。

Objective To study the development and incidence of acute rejection in acute rejection model of DA - to - Lew rat liver trans- plantation. Methods The rats were divided into isogeneic （ Lewis - Lewis） group （ n = 24） and allogeneic （ DA - Lewis） group （ n = 24）. On day 3, 5, 7 and day 10 after surgery, three recipient rats of each group were randomly sacrificed respectively. Pathological changes of the liver and the levels of aspartate aminotransferase （AST）, total bilirubin and eytokines were observed. Results There were significant changes in the general per- formance of the rats, the function and pathological changes of the liver and cytokines level in all the time points of observation between allogeneic group and isogeneic group. Severe acute rejection was observed on day 7 after liver transplantation. Conclusion The study suggests severe acute rejection develops＇ on day 7 after surgery in acute rejection model of DA - to - Lew rat liver transplantation and the severity of acute rejection can be evaluated at this time point.