小鼠结肠癌完整组织块原位种植及肝转移模型的建立方法

Rapid establishment of orthotopic implantation tumor and metastasis model of colon cancer in mice

[目的]研究快速建立模拟临床过程的小鼠结肠癌原位移植瘤及肝转移模型方法。[方法]采用小鼠结肠癌细胞CT26接种于裸鼠皮下,形成稳定传代的皮下种植瘤。再取该肿瘤组织块,用原位医用胶黏合法种植于BALB/c小鼠盲肠壁,建立模拟于临床的结肠癌肝转移模型,定时观察小鼠全身情况,分不同时期处死,观察其生长及转移特性。[结果]原位种植成瘤率100%,术后3周肝转移率100%,4周后动物消瘦,萎靡,行动迟缓,反应迟钝,部分可见腹水形成,濒临死亡。中位生存期29d。[结论]小鼠结肠癌完整组织块通过"生物胶黏贴法"原位种植BALB/c小鼠盲肠壁,速度快,方法简便,能较好的重现结肠癌临床转移的过程,为人类结肠癌生长、肝转移机制及干预肝转移治疗的研究提供一种较理想的动物模型。

[Objective]To establish an orthotopic implantation and metastasis model of colon cancer in mice rapidly. [Methods]Tumor cell line CT26 of mice colon adenocarcinoma was inocula ted subcutaneously into nude mice to develop implantation tumor. Histologically intact tumor tis- sue was then harvested and implanted to the colon wall of mice to set up a model similar to human colon cancer. The formation of implanted tumor rate, local tumor growth characteristics, and liver metastasis rates were examined each week, Five mice were killed after two weeks, three weeks, and four weeks,respectively. [Results]A 100~//00 implanted tumor rate was obtained in this mod- el. The incidences of liver metastases were 100~/00 after 3 weeks. Emaciation and exhaustion of the mice were presented after 4 weeks. The median survival time of the tumor-bearing mice was 29 days. i-Conclusion]The ortbotopic implantation tumor and metastasis model provides useful tools for the study of mechanism of metastasis and its treatment of human colon cancer.