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西洛酰胺对绵羊卵母细胞核成熟及后期胚胎发育的影响 被引量：3

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摘要 本实验研究不同浓度西洛酰胺(Cilostamide)对绵羊卵母细胞核成熟的抑制效果,并探讨FSH对Cilostamide抑制效果的影响,以及延迟卵母细胞核成熟对随后胚胎发育能力的影响,旨在探索提高绵羊体外胚胎发育能力的新方法。结果表明:5μmol/L的Cilostamide为较合适的添加浓度,使卵母细胞核成熟延迟约4 h;FSH与Cilostamide对抑制核成熟起到协同作用;5μmol/L Cilostamide处理可使绵羊卵母细胞孤雌激活后囊胚率达到40.4%,显著高于对照组29.0%的囊胚率(P<0.05)。
作者 胡媛媛 田树军 温兵强 孙树春 赵驻军 祁昕
机构地区 河北农业大学动物科技学院 河北省牛羊胚胎工程技术研究中心
出处 《中国畜牧杂志》 CAS 北大核心 2012年第15期23-26,共4页 Chinese Journal of Animal Science
基金 国家科技支撑计划(2011BAD19B02) 教育部科学技术研究重点项目(211018)
关键词 卵母细胞 西洛酰胺 促卵泡素 体外成熟 绵羊
分类号 S826.3 [农业科学—畜牧学]
  • 相关文献

参考文献10

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二级参考文献12

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共引文献1

同被引文献26

  • 1卜淑敏,周波,夏国良.3种磷酸二酯酶对小鼠卵母细胞自发成熟的影响[J].中国农业大学学报,2007,12(5):1-4. 被引量：2
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  • 9魏凤,吕素芳,郭广君,沈志强,扈俊波.动物胚胎生物技术研究进展[J].上海畜牧兽医通讯,2008(1):6-7. 被引量：2
  • 10张红琴,周欢敏,李宝山,张东,朱琳.不同激活方法对绵羊体外成熟卵母细胞孤雌生殖的影响[J].内蒙古农业大学学报（自然科学版）,2008,29(1):78-81. 被引量：3

引证文献3

二级引证文献1

中国畜牧杂志
2012年 第15期

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