氧化损伤产物8-OHdG、4-HNE、NTY在成年小鼠卵巢组织中的增龄变化

Expression of oxidative damage production(8-OHdG, 4-HNE, NTY) in ovarian tissue of adult mice

目的:探讨DNA、脂质和蛋白质氧化损伤产物在成年小鼠卵巢组织中的增龄变化。方法:选用12、24、48、75周龄自然衰老C57BL/6小鼠为模型。采用免疫组织化学方法和蛋白印迹技术检测各年龄组小鼠卵巢中8羟基脱氧鸟苷(8-hydroxy-2-deoxyguanosine,8-OHdG)、4羟基壬烯醛(4-hydroxynonenal,4-HNE)、硝基酪氨酸(nitrotyrosine,NTY)的表达变化情况。结果:8-OHdG、4-HNE、NTY在卵巢间质细胞随增龄表达增加(P<0.05)。12周龄组3种物质的表达均明显低于其他各组,差异有显著统计学意义(P<0.01);24周龄含量低于48、75周龄组,差异有显著统计学意义(P<0.05);48周龄组与75周龄组比较,差异无统计学意义(P>0.05)。而各年龄组卵泡膜细胞、颗粒细胞及卵母细胞中未见明显变化。结论:氧化损伤在卵巢间质细胞中随增龄表现明显,间质细胞氧化损伤可能是导致卵巢衰老的原因之一。

Objective To investigate expression of oxidative damage productions in ovarian tissue of adult mice. Methods Mouse model was established using 12 useeks, 24 weeks, 48 weeks, 75 weeks C57BL/6 female mice. Immunohistochemistry and western bloting were used to detect 8-hydroxy-2-deoxyguanosine （8-OHdG）, 4- hydroxynonenal （4-HNE） and nitrotyrosine （NTY） which were respectively used to localize and evaluate oxidative damage of lipid, protein and DNA. Results The level of 8-OHdG, 4-HNE, NTY in ovarian interstitial tissue significantly increased with age （P 〈 0.05）. The expression of them in 12 weeks group was the lowest among the four groups （P 〈 0.01 ） ; 24 weeks group expression level was lower than that of 48 weeks group and 75 weeks group （P 〈 0.05） ; but no significant difference between 48 weeks and 75 weeks group （P 〉 0.05）. There was no significant discrepancy of theca cells, granulosa cells and oocyte cells among four groups. Conclusions Oxidative damage significantly increased in ovarian interstitial tissue with age. The age-related oxidative damage of ovarian interstitial tissue may play an important role in ovarian aging.