五味子乙素增强乳腺癌小鼠对紫杉醇敏感性的实验研究

Experimental research on Schizandrin B enhancing sensitivity to paclitaxel on rats with mammary carcinoma

目的:探讨五味子乙素能否增强乳腺癌小鼠对紫杉醇(PTX)的敏感性。方法:将BALB/c小鼠50只随机分为5组:正常(NC)组、模型对照(MC)组、紫杉醇(PTX)组、五味子乙素(SchB)组、五味子乙素+紫杉醇(SchB+PTX)组。SchB10mg/kg,腹腔注射,每日1次;PTX10mg/kg,腹腔注射,隔日1次。给药22d,以体重、肿瘤体积、肿瘤生长抑制率及脏器指数为观察指标。结果:PTX组、SchB+PTX组的体重,与MC组比较,均从给药第7天开始下降且均具有统计学差异;SchB+PTX组与PTX组比较,体重略有增长,但无统计学差异。PTX组的肿瘤生长抑制率为15.7%,SchB+PTX组为25.9%。PTX组和SchB+PTX组,与MC组比较,脾脏指数均降低(P=0.008;0.000);SchB+PTX组与PTX组比较,脾脏指数有降低趋势,但无统计学差异。SchB+PTX组与PTX组比较,胸腺指数和肾上腺指数均较大(P=0.000;0.011)。结论:SchB和PTX联用,可增强PTX抗肿瘤作用,并减轻PTX对小鼠的副作用。

Objective To investigate the effects of Schizandrin B （Sch B） on enhancing sensitivity to paclitaxel （PTX） on rats with mammary carcinoma. Methods Fifty BALB/c rats were randomly divided into five groups, 10 animals of each group： a normal control （NC） group, model control （MC） group, PTX group, Sch B group and PTX ＋ Sch B group. Sch B was administered to the rats by intraperitoneal injection at a dose of 10mg/kg once per day for 22 days. while PTX was every 2 days. The body weight, gross tumor volume, inhibition rate of tumor growth and organ index were observed. Results Compared with the MC group, the body weight in both the PTX group and Sch B ＋ PTX group began to decrease since the 7th day of administration, the difference in weight was significant by statistics. Compared with the PTX group, the body weight in the Sch B ＋ PTX group was elevated, but the difference was not significant. The inhibition rate in the PTX group was 15.7% and that in the Sch B ＋ PTX group 25.9%. Compared with the MC group, the spleen index in PTX group and the Sch B ＋ PTX group was significantly lower （P = 0.008 and 0.000, respectively）. Compared with the PTX group, the spleen index in the Sch B ＋ PTX group was lower, but the difference was not significant. Compared with the PTX group, the thyroid and adrenal gland index in the Sch B ＋ PTX group was significantly elevated （P = 0.000 and 0.011, respectively）. Conclusions Combination of Sch B and PTX can enhance anti-tumor effects and relieve side effects of PTX on rats with mammary carcinoma.