利用GC-MS技术分析油橄榄叶挥发油化学成分

Analysis of the Chemical Composition of Essential Oil from Olive Leaf by GC-MS Method

本文采用同时蒸馏萃取法(SDE)提取油橄榄叶挥发油化学成分,利用气相色谱-质谱(GC-MS)联用方法分析并用峰面积归一化法得出各化学成分在挥发油中的百分含量。结果从油橄榄叶挥发油中分离出62各色谱峰,鉴定出56个化合物,占挥发性组分总量的95.6%。其中主要成分为α-芹子烯(13.13%)、β-芹子烯(10.15%)、β-石竹烯(14.29%)、橙花叔醇(6.72%)、α-石竹烯(6.60%)、3-己烯-1-醇(5.03%)、苯乙醛(5.61%)等。

The volatile oil of olive leaf obtained by simultaneous distillation extraction was analyzed by gas chromatography-mass spectrometry（GC-MS）,and the content of each component was determined by using the normalization method.Among the 62 constituents,56 components were identified,accounting for 95.6% of the total volatile oil.The major constituents were α-selinene（13.13%）,β-selinene（10.15%）,β-caryophyllene（14.29%）,nerolidol（6.72%）,α-caryophyllene（6.60%）,3-hexene-1-ol（5.03%）,and phenylacetaldehyde（5.61%）.