悬浮少白细胞红细胞在保存期溶血性指标的比较

Comparison of Haemolysis in Red Blood Cells Suspension with Reduced Leukocyte at Different Times during the Storage period

目的对悬浮少白细胞红细胞制品在保存期不同时间段溶血性指标进行比较,同时制定该制品溶血性指标在保存期末的内控标准,以保证提供临床合格的血液制品。方法用3家不同厂家的去白细胞滤器血袋对90例全血进行去白,制备成悬浮少白细胞红细胞制品。分别在第0天、第7天、第21天、第28天和第35天对悬浮少白细胞红细胞制品的游离血红蛋白含量进行检测。结果 3家不同厂家的去白滤器制备悬浮少白细胞红细胞制品,其游离血红蛋白数值(g/L)在第0天、第7天和第35天分别为0.13±0.08、0.06±0.05、0.09±0.06;0.69±0.28、0.72±0.30、0.65±0.24和1.03±0.36、1.18±0.38、1.05±0.38。结论通过对悬浮少白细胞红细胞制品在保存期不同时间段溶血性指标的检测,分析影响该制品溶血的因素并加以控制。

Objective To compare haemolysis in red blood cells suspension with reduced leukocyte at different times during the storage period, develop quality internal enterprise standards of haemolysis in the product at the end of the stor- age period, and provide clinical blood products. Method 90 cases of red blood cells suspension with reducted leukocyte were prepared with three different manufacturers＇ leukocyte reduction sets. At day 0, 7, 21, 28 and 35, the level of free hemoglobin in red blood cells suspension were detected. Results The value of free hemoglobin （g/L） in the red blood ceils suspension using three different manufacturers＇ leukocyte reduction sets at day 0, 7 days and 35 days respectively were 0.13±0.08, 0.06±0.05, 0. 09±0. 06; 0.69±0.28, 0.72±0.30, 0.65±0.24 and 1.03±0.36, 1.182±0.38, 1.05±0. 38. Conclusion We analyzed the factors affecting hemolysis of the red blood cells suspension with reduced leukocyte products by analyzing haemolysis in the products at different times during the storage period, and strengthen the control of these factors.