IGF2BP2基因rs4402960多态性与内蒙古地区汉族人群2型糖尿病的相关性研究

Association of IGF2BP2 rs4402960 genotype with type 2 diabetes susceptibility in Han population living in Inner Mongolia region

目的探讨内蒙古地区汉族人群胰岛素样生长因子2 mRNA结合蛋白(IGF2BP2)基因rs4402960单核苷酸多态性的等位基因和基因型频率分布与2型糖尿病(type 2 diabetes,T2DM)的相关性。方法采用等位基因特异性聚合酶链反应(AS-PCR)对360例内蒙古地区汉族人(T2DM组166例,正常对照组194例)rs4402960进行基因分型。结果 T2DM组中rs4402960的T等位基因频率和TT、GT基因型频率分别为41.6%、12.1%和59.0%,均高于正常对照组的10.6%、3.6%和13.9%(P均<0.05);而T2DM组的GG基因型频率为28.9%,低于正常对照组的82.5%(P<0.05)。携带T等位基因是患T2DM的危险因素(OR=6.02,95%CI=4.075～8.895)。结论 IGF2BP2基因rs4402960多态性位点的T等位基因可能是T2DM的风险等位基因,该位点G/T多态性与内蒙古地区汉族人群T2DM具有相关性,可能是内蒙古地区汉族人T2DM的易感基因之一。

Objective To study the association of alleles and genotype frequencies of rs4402960 in insulin-like growth factor 2 mRNA binding protein 2（IGF2BP2） gene with type 2 diabetes mellitus（T2DM） in Han population of Inner Mongolia region.Methods Allele-specific polymerase chain reaction was used to genotype the rs4402960 polymorphism of IGF2BP2 in 360 Han population living in the Inner Mongolia region,including 166 patients with T2DM（T2DM group） and 194 normal controls（NC group）.Results The frequencies of T allele,TT and GT genotype of rs4402960 in T2 DM group were 41.6%,12.1% and 59.0%,respectively,which were significantly higher than those in the NC group（10.6%,3.6% and 13.9%,all P〈0.05）.The GG genotype frequency was 28.9% in T2DM group,being significantly lower than that in the NC group（82.5%,P〈0.05）.Carrying T allele was a risk factor of T2DM（OR = 6.02,95% CI = 4.075-8.895）.Conclusion T allele of rs4402960 polymorphism in IGF2BP2 gene might be a risk factor of T2DM,and G/T polymorphism is associated with T2DM.IGF2BP2 rs4402960 might be a susceptible gene of T2DM in Han population living in Inner Mongolia region.