3株柔嫩艾美耳球虫28S rRNA基因部分序列的扩增与分析

PCR Amplification and Sequence Analysis of Partial 28S rRNA Sequence of three Eimeria tellena Strains

本研究应用PCR技术扩增来自广东的3株柔嫩艾美耳球虫的28SrRNA基因部分序列,并与GenBankTM登录的柔嫩艾美耳球虫、堆型艾美耳球虫、鼠肉孢子虫和刚地弓形虫虫株的相应序列进行比对分析。试验结果显示,柔嫩艾美耳球虫3个样品均获得1172bp的28SrRNA基因部分有效序列,不同虫株序列没有差异,与GenBankTM登录的柔嫩艾美耳球虫相应序列只有一个碱基差异,显示种内序列高度保守,而与堆型艾美耳球虫、鼠肉孢子虫、刚地弓形虫相应的序列存在不同程度的差异。结果表明,28SrRNA基因部分序列可作为研究艾美耳球虫种间及其他顶复门原虫遗传变异的标记。

The partial 28S ribosomal RNA sequences of three different Eimeria tellena strains isolated from Guangdong province were amplified by polymerase chain reaction （PCR）, and compared with relevant sequences of E. tellena, E. acervuli- ha, Sarcocystis muris , T. gondii available in GenBankTM. The results showed that the partial 28S rRNA sequence of three E. tellena strains was 1172 bp in length with no nucleotide difference in each other, and only one base difference found with E. tellena in GenBankTM , revealing highly conservative within E. tellena. There were distinct sequence differences found com- paring with E. acervulina, S. muris and T. gondii, which demonstrated 28S rRNA could be used as a genetic marker for the population genetic studies on different species of Eimeria and other apicomplexan parasites.