ACVR1基因对京海黄鸡生长和繁殖性状的遗传效应研究

Genetic Effects of ACVR1 Gene on Growth and Reproductive Performance in Jinghai Yellow Chicken

以京海黄鸡为材料,采用PCR-SSCP和DNA测序技术对ACVR1基因外显子多态性进行研究。结果表明,ACVR1基因20190bp处发生G→A突变,检测到AA、AB和BB 3种基因型。A等位基因频率为0.9075,B等位基因频率为0.0925。基因型与生长和繁殖性状的关联性分析发现,BB基因型鸡的开产体重极显著高于AA基因型鸡(P<0.01),AA基因型鸡300日龄体重显著高于BB基因型鸡(P<0.05)。由此初步推断,ACVR1基因可能对京海黄鸡的产蛋和发育有一定的影响,A为体重的优势基因,B为产蛋的优势基因。

In this study, Jinghai Yellow chicken was used to analyze ACVR1 gene polymorphisms by PCR-SSCP and DNA sequencing method. As a result, there was one mutation site（G20190A） in Jinghai Yellow chicken. Three genotypes were de- tected（AA, AB, BB）, and the frequencies of allele A and B were 0. 9075 and 0. 0925. For the reproductive performance, BB genotype in open weight was significantly higher than AA genotype （P〈0. 01）, however AA genotype in 300 days of age weight was significantly higher than BB genotype （P〈0.05）. It implied that ACVR1 gene might have a fixed effect on growth and reproductive effect in Jinghai Yellow chicken.