应用体内诱导抗原技术筛选病原菌体内表达基因的研究进展

Overview of Identification of Ivi Genes Uniquely Expressed during the Infection with Pathogenic Bacteria Using IVIAT

病原菌体内诱导基因与其在体内的生存和致病过程密切相关,体内诱导抗原技术(IVIAT)已广泛应用于筛选体内诱导基因的研究。IVIAT不使用动物模型,采用经病原菌体外培养抗原吸附处理的感染动物血清来检测病原菌的原核表达文库,对体内诱导基因进行筛选。利用该方法可以快速、简单的筛选到体内诱导基因,有助于病原菌致病机制的研究,同时也能够为目标病原菌抗菌药物、诊断试剂及疫苗设计的开发研究提供理论依据。文章就IVIAT的原理、兽医致病病原菌中的应用及应用前景作一简略的概述。

An overview of in vivo induced antigen technology （IVIAT） will be presented, including a discussion of the ad- vantages and limitations of this method. In vivo induced antigen technology （IVIAT） is a technique that identifies pathogen an tigens that are immunogenic and expressed in vivo during animal infection. Over 20 different microbial pathogens have been or are known to be currently studied with this method. IVIAT is complementary to other techniques that identify genes and their products expressed in vivo. Genes and gene pathways identified by IVIAT may play roles in virulence or pathogenesis during animal infection, and may be appropriate for inclusion in therapeutic, vaccine or diagnostic applications.