摘要
黑素皮质素受体-4(melanocortin receptor-4,MC4R)、黑素皮质素受体-3(melanocortin receptor-3,MC3R)、瘦素(Leptin)和缩胆囊素受体(cholecystokinin A receptor,CCKAR)基因均是与采食量相关的功能基因,为了研究这4个基因之间可能存在的互作关系,本研究采用RNAi方法,将体外培养的成纤维细胞中的MC4R基因沉默,然后采用Real-time PCR方法对Leptin、MC3R和CCKAR基因的表达变化情况进行了研究。结果表明,MC4R被沉默后,Leptin、MC3R和CCKAR的表达水平均没有发生显著变化。
MC4R, MC3R, Leptin and CCKAR are all associated with feed intake. To analysis the potential relationship of these four genes, MC4R gene was silenced in fibroblasts by RNAi method, then Real-time PCR method was used to detect the expression changes of Leptin, MC3R and CCKAR. The results showed that, after MC4R was silenced, Leptin, MC3R and MC4R expression level were not changed.
出处
《中国畜牧兽医》
CAS
北大核心
2012年第7期147-150,共4页
China Animal Husbandry & Veterinary Medicine
基金
转基因生物新品种培育重大专项(2008ZX08006-003)
