电针对SAMP8小鼠海马线粒体呼吸链功能的影响

Effects of electroacupuncture on the function of respiration chain in hippocampal mitochondria of SAMP8 mice

目的:从线粒体角度探讨电针治疗阿尔茨海默病(AD)的作用机制。方法:选取8月龄SAMP8小鼠12只,随机分为模型组6只,电针组6只,另选取6只8月龄的SAMR1小鼠为对照组。电针组电针"百会""大椎""肾俞""太溪"穴,每日1次,每次20min,10次为一疗程,共治疗3个疗程;其他两组不做治疗干预,只进行相同方式、相同时间和相同程度的抓取和小鼠固定器固定。疗程结束后,采用Morris水迷宫检测小鼠学习记忆能力,分光光度法检测海马线粒体呼吸链酶复合物的活性,反相高效液相色谱法检测细胞三磷酸腺苷(ATP)水平。结果:与对照组相比,模型组小鼠平均逃避潜伏期明显延长,原平台象限停留时间缩短,海马线粒体呼吸链酶复合物Ⅰ、Ⅱ、Ⅲ和Ⅳ活性降低,细胞ATP含量减少;模型组比较,电针组平均逃避潜伏期缩短,原平台象限停留时间延长,海马线粒体呼吸链酶复合物Ⅰ、Ⅱ、Ⅲ和Ⅳ活性升高,细胞ATP含量增加。结论:电针能够提高小鼠海马线粒体呼吸链酶复合物活性及细胞ATP含量,改善线粒体功能,可能是治疗AD的机制之一。

Objective To investigate the mechanism of electroacupuncture in treatment of Alzheimer’s disease（AD） from the perspective of mitochondria.Methods Tweleve SAMP8mice aged 8months were randomly divided into a model group（n=6）and an electroacupuncture group（n=6）,and six SAMR1mice aged 8months were selected as a control group.The electroacupuncture group was treated with electroacupuncture at＂Baihui＂（GV 20）,＂Dazhui＂（GV 14）,＂Shenshu＂（BL 23）and＂Taixi＂（KI 3）for 20min,once each day,10days for a course,and lasted for 3courses.The other two groups were grasped and fixed in the same way at the same time without the other treatment.After the end of treatment,the learning and memory abilities of the mice were measured by Morris water maze,the activity of hippocampal mitochondrid respiratory chain enzyme complex was performed by spectrophotometry,and the levels of adenosine triphosphate（ATP）were detected by a re-verse-phase high performance liquid chromatography（HPLC）method.Results Compared with the control group,the average escape latency significantly lengthened,the residence time on the plateau phase shortened,the activity of respiratory chain enzyme complexeⅠ,Ⅱ,Ⅲ,andⅣwas decreased,and ATP concentration was decreased in the model group.Compared with the model group,the average escape latency significantly shortened,the residence time on the plateau phase lengthened,the activity of hippocampal mitochondrid respiratory chain complexesⅠ,Ⅱ,Ⅲ,andⅣwas significantly increased,and ATP concentration was increased in the electroacupuncture group.Conclusion Electroacupuncture can increase the activity of hip-pocampal mitochondrid respiratory chain enzyme complexe and ATP concentration and improve mitochondrial function,which may be one of underlying mechanisms of electroacupuncture in treatment of AD.