肺炎衣原体诱发肺癌模型的建立

Study on Lung Cancer Model Induced by Chlamydia Pneumoniae

目的:建立肺炎衣原体(Chlamydia pneumoniae,Cpn)诱发大鼠肺癌模型。方法:采用Cpn菌株TW-183感染的方法以及TW-183感染联合苯并芘(Benzopyrene,Bp)的方法建立肺癌Wistar大鼠模型。采用微量免疫荧光法检测大鼠血清中Cpn特异性抗体,采用聚合酶链式反应的方法检测大鼠肺癌组织中的Cpn DNA,采用酶联免疫吸附法检测大鼠肺癌组织中的Cpn特异性抗原。结果:单独Cpn感染大鼠的Cpn感染率为72.9%(35/48),Cpn感染联合Bp大鼠的Cpn感染率为76.7%(33/43);两组的肺癌发生率分别为14.6%(7/48)和44.2%(19/43),均显著高于对照小鼠的肺癌发生率(P=0.012,P<0.001)。结论:本研究成功建立Cpn感染诱发大鼠肺癌模型。

Objective： To build a rat model of lung cancer induced by Chlamydia pneumoniae（Cpn） infection. Methods： Cpn （TW 183） was injected into the lungs of the rats with or without the combination of Benzopyrene（Bp）. Cpn-antibodies（Cpn- IgA, -IgG and -IgM） in rat serum were detected by the method of microimmunofluorescence（MIF）. The Cpn-DNA and Cpw Ag in lung cancer tissues were detected by polymerase chain reaction（PCR）and enzyme-linked immunosorbent assay（ELISA）, respectively. Results： The incidences of Cpn infection were 72.9 % （35/48） in rats injected with Cpn and 76.7% （33/43） in rats injected with Cpn and Bp; and the incidences of lung cancer in them were 14.6%（7/48） and 44.2%（19/43） , respectively, all of which were significantly higher than the controls（P = 0. 012, P〈0. 001, respectively）. Conclusions： A rat model of lung cancer induced by Chlamydia pneumoniae infection has been successfully built.