合并多囊卵巢综合征产妇胎盘肥胖相关基因多态性研究

Polymorphism of Fat Mass and Obesity Associated Gene in the Placenta Tissues from Patients with Polycystic Ovarian Syndrome

目的:研究多囊卵巢综合征(polycystic ovarian syndrome,PCOS)患者胎盘肥胖相关基因(fat mass and obesity associated gene,FTO)rs1421085(C/T)的单核苷酸多态性(single nucleotide polymorphism,SNP),探讨PCOS患者子代再次发生P-COS及相关疾病的风险。方法:运用聚合酶链反应-限制性片段长度多态性(Polymerase chain reaction-restriction fragmentlength polymorphism,PCR-RELF)分析及基因测序的方法,在26例PCOS患者(研究组)和27例健康志愿者(对照组)的胎盘组织中,检测FTO基因rs1421085(C/T)位点的SNP。结果:研究组胎盘FTO基因rs1421085(C/T)等位基因频率与对照组比较有显著差异(P=0.0072)。两组基因型频率相比也有显著差异(P=0.0285)。结论:PCOS患者胎盘组织中FTO基因rs1421085(C/T)位点SNP变异的发生率较正常人群高,PCOS患者后代再次发生PCOS及相关疾病的风险可能会增加。

Objective：To study the polymorphism of fat mass and obesity associated gene（FTO） rs1421085 （C/T） in placenta tissues from patients with polycystic ovarian syndrome（PLOS）, in order to explore the risk of PC.OS and relevant diseases in offspring of PCOS patients. Methods：A total of 28 cases of PCOS and 27 healthy volunteers were enrolled. Polymerase chain reaction-restriction fragment length polymorphism （PCR-RELF） and gene sequencing were used tc detect the single nucleotide polymorphism（SNP） of FTO rs1421085 （C/T）. Results： The allele frequency of FTO rs1421085 {C/T） was significantly dif- ferent between study group and control group （P = 0. 0072）. The frequency of genotype was signiflcantly different between the two groups （P = 0. 0285）. Conclusions： The SNP of FTO rs1421085（C/T） variation rate is significantly increased in PCOS pa- tients. It reveals that the offspring of PCOS women may have higher risk in suffering from PCOS and relevant diseases.