摘要
睾丸特异性丝氨酸/苏氨酸蛋白激酶(Testis-SpecificSerine/Threonine Kinases,TSSKs)可能在精子发生和(或)精子功能调节中起着重要作用,该文研究克隆并表达了小鼠Tssk1和Tssk2基因,纯化得到了Tssk1和Tssk2蛋白激酶,经Western blotting分析,Tssk1和Tssk2皆存在于小鼠和人的成熟精子中。免疫组化的结果显示,Tssk1分布于小鼠的头部(顶体)及整个尾部,Tssk2主要分布在小鼠精子头部顶体后的区域;获能前后的小鼠精子其Tssk1及Tssk2分布模式未发生改变,小鼠Tssk2在诱发顶体反应前后精子中的分布模式也无变化。然而,原来存在于顶体的Tssk1在诱发顶体反应后由于顶体的丢失而未能检出其信号,但尾部的信号不受影响。在人精子中,Tssk1分布区域为颈部及尾部,Tssk2则分布于赤道板的位置。研究结果提示,Tssk1和Tssk2可能对精子功能具有重要调节作用。
We sought to experimentally verify if testis specific serine/threonine kinases （Tssks） play a role in spermatogenesis and/or the regulation of sperm fimction. Purified Tssk proteins were obtained based on cloning and expression of mouse Tsskl and Tssk2. Tsskl and Tssk2 were detected in mature mouse and human sperm by western blotting. Immunofluorescence indicated that Tsskl is distributed in the acrosome and the entire flagellum of mouse sperm while Tssk2 was mainly distributed in post-acrosomal region. There was no alteration in the distribution pattern of Tsskl and Tssk2 in non-capacitated and capacitated sperm. Tssk2 distribution remained unchanged after induced acrosome reaction but no signals were detected in the acrosome for Tsskl, which was present before the acrosome reaction, though signals in flagellum were undisturbed. In human sperm, Tsskl was found in neck and flagellum while Tssk2 was found in the equatorial region. Our results suggest Tsskl and/or Tssk2 do play an important role（s） in the regulation of sperm function.
基金
国家自然科学基金专项基金(31040055)
