调节性T细胞和Thl7细胞在新型小鼠重型再生障碍性贫血模型中的变化

The changes of regulatory T cells and Thl7 cells in a novel mouse severe aplastic anemia model

目的探讨调节性T细胞和Thl7细胞在干扰素一1（IFN一^y）联合白消安诱导的新型小鼠重型再生障碍性贫血（AA）模型中的变化，从免疫学水平上论证该模型合理性。方法应用IFN一1腹腔注射联合白消安灌胃诱导建立BALB／c小鼠重型AA模型（联合组），以白消安组、IFN一-,／组和正常组为对照，分别收集各组小鼠的外周血和脾脏中的单个核细胞，采用流式细胞术检测各组小鼠外周血和脾脏中CIM’CD25’FOXP3’调节性T细胞和Thl7细胞的比例变化，并逐层分析比较。结果联合组小鼠外周血和脾脏的调节性T细胞的百分比分别为（3．19±0．76）％和（4．77±1．05）％，较正常组、白消安组、IFN-1组均降低，差异有统计学意义（P值均〈0．01）；而Thl7细胞百分比分别为（2．07±0．12）％和（3．18±0．46）％，较其他三组均升高，差异有统计学意义（P值分别〈0．05和0．叭）。结论IFN-γ联合白消安诱导建立的重型AA小鼠调节性T细胞数量降低和Thl7细胞升高，提示该小鼠重型AA模型可能更接近并模拟了人类免疫介导的骨髓造血功能损伤。

Objective To explore the changes of regulatory T（Treg） cells and Thl7 cells in a novel mouse severe aplastic anemia （SAA） model induced by interferon-＇y （IFN-γ） combined with bnsulphan （BU） , and to demonstrate the rationality of the model in immunology level. Methods The BALB/c female mice SAA model was constracted by intraperitoneal injection with IFN-~, and intragastric administration with BU （combined group）, with BU group, IFN-~ group and normal group as controls. After collecting the mono- nuclear cells in the peripheral blood （PB） and spleen of mice in each group, the percentage of CD4 ＋ CD25 ＋ FOXP3 ＋ Treg cells and Thl7 cells in the mononuelear cells were detected by flow cytometry（ FCM）, and to analyze the changes. Results The percentage of the Treg cells in PB and spleen was （3.19 -＋ O. 76）% and （4.77 ＋ 1.05 ） % respectively in combined group, being significantly lower than in other three groups （ all P 〈 0.01 ）. The percentage of the Thl 7 cells in PB and spleen was （2.07 ＋ 0.12） % and （ 3.18 ＋ 0.46） % respectively in combined group, being significantly higher than that in other three groups （ P 〈 0.05 and O. 01, respectively）. Conclusions Lower Treg cells and higher Thl7 cells was found in the novel mouse SAA model induced by IFN-~, combined with BU, which demonstrates that this SAA model may be more close to the human immune-mediated marrow failure.