HBcAg和HBeAg对人树突状细胞成熟和功能的影响

Influence of hepatitis B core antigen and e antigen on the maturation and function of human dendritic cells

目的观察乙型肝炎核心抗原(hepatitis B core antigen,HBcAg)和乙型肝炎e抗原(hepatits B e antigen,HBeAg)对人树突状细胞的影响。方法分离20例健康者外周抗凝血淋巴细胞,将贴壁细胞加重组人白细胞介素4和重组人粒-巨噬细胞集落刺激因子培养至第7天,将树突状细胞分为HBcAg组、HBeAg组和树突状细胞对照组,分别加入HBcAg、HBeAg对树突状细胞刺激培养至第10天,检测刺激培养后细胞表型与分泌的细胞因子及其对树突状细胞体外诱导的淋巴细胞增殖效应和淋巴细胞毒活性的影响。结果 HBcAg组、HBeAg组树突状细胞表面分子(CD83,CD86,HLA-ABC)水平和分泌干扰素-γ、白细胞介素-12p70水平、体外诱导淋巴细胞增殖效应及淋巴细胞毒活性与对照组比较,差异有统计学意义(P<0.01);HBcAg组增强树突状细胞的分子表达、分泌白细胞介素-12p70及体外诱导淋巴细胞毒活性与HBeAg组比较差异有统计学意义(P<0.01)。结论 HBcAg和HBeAg可增加人树突状细胞表达成熟活化的分子、分泌细胞因子,增强树突状细胞体外诱导淋巴细胞增殖效应及淋巴细胞毒活性。

Objective To explore the influence of hepatitis B core antigen （HBcAg） and hepatitis B e antigen （HBeAg） on the maturation and function of human dendritic cells. Methods The dendritic cells were generated from peripheral blood mononuclear cells joined with interleukin-4 and granulocyte macrophage colony stimulating factor derived from 20 healthy donors and then pulsed with HBcAg and HBeAg respectively on the seventh day. The cell surface molecules including CD83, CD86 and HLA-ABC in HBcAg group, HBeAg group and control group were analyzed with flow cytometry on the tenth day. The lymphocyte proliferation, the cytotoxic activity, as well as the IFN-y and IL-12 p70 secretion were observed. Results The levels of maturation-associated phenotypic markers （human leucocyte antigen HLA- ABC, CD83 and CD86）, the IFN-γ and IL-12p70 secretion, the lymphocyte proliferation, and the cytotoxic activity showed significant differences in HBcAg group and HBeAg group compared with control group （P〈0. 05）. There were significant differences in the levels of maturation-associated phenotypic markers, IL-12p70 secretion and the cytotoxic activity between HBcAg group and HBeAg group （P 〈 0.01）. Conclusion HBcAg and HBeAg can enhance the maturation and secretion of human dendritic cells, and can improve lympholeukocyte multiplication and cytotoxic T lymphocyte activity.