吉兰-巴雷综合征空肠弯曲菌感染诱导巴马小型猪致周围神经病动物模型的建立

Establishment of animal model of Bama miniature pigs for peripheral nerve disease: infected by Guillain-Barré syndrome-induced campylobacter jejuni

目的使用致吉兰-巴雷综合征的空肠弯曲菌活菌诱导的巴马小型猪建立周围神经病动物模型。方法使用吉兰-巴雷综合征病人粪便中分离的空肠弯曲菌,微需氧培养后口服攻毒30只巴马小型猪,建立周围神经病动物模型。结果实验组在攻毒后14～16天出现临床症状,两周内解剖。临床症状周围神经病重症的占40.9%(9/22),出现周围神经病症状的占81.8%(18/22)。病理学检查锇酸染色可见卵圆体结构,镀银坚固蓝染色见轴索断裂,未见脱髓鞘。结论本实验通过致吉兰-巴雷综合征的空肠弯曲菌活菌构建了巴马小型猪周围神经病动物模型,模拟了自然条件下侵染致病的过程,为研究周围神经病的发病机理提供了大型动物模型。

Objective exploring campylobacter jejuni which induce Gnillain-Barre syndrome （GBS） to establish peripheral nerve disease animal models of Bama miniature pigs. Methods Strain lulei （penner O19） was isolated from the feces of GBS patient. Bama miniature pigs were oral with microaerobic cultured stain lulei to established animal models. MRI testing data of brain tissue were collected after anesthesia. Using histopathological methods to test peripheral nerve in two weeks of come on disease. Results The experiment group had clinical symptoms in 14-16 days. The anatomy results showed that 8l. 8% had symptoms in which 40.9% were significant. Ovoid bodies could be detected in Osmium Tetroxide Stain and axonal injury could be detected in Luxol fast blue stain. The demyelination was not identified. Besides, parts of the samples had edema in cerebellum or muscle. Conclusion The animal model of Bama miniature pig is established successfully. This model mimicks the infection pathway and provides a sensitive model to study peripheral nerve pathngenesis.