C反应蛋白基因多态性与缺血性脑卒中的关系

Association between C-reactive protein gene polymorphism and cerebral infarction

目的探讨超敏C反应蛋白(hs-CRP)水平及rs1130864基因多态性与脑梗死(CI)的相关性。方法采用病例对照研究,对105例脑梗死患者和121例健康对照者进行研究。采用免疫透射比浊法测定hs-CRP,用多聚酶链式反应(PCR)和限制性片段长度多态性测定rs1130864基因多态性。患者入院后立即用美国国立卫生院神经功能缺损评分(National Institutes of Health Stroke Scale,NIHSS)对其进行神经功能测评。结果脑梗死组与对照组rs1130864基因型频率分布统计学无显著性差异(P>0.05)。rs1130864与脑梗死病情无关联。脑梗死多因素回归方程的主要危险因素包括高血压史、糖尿病史、吸烟史、HDL-C,hs-CRP水平。结论 rs1130864(1444C>T)基因多态性与脑梗死无关联。

Objective To explore the relationship between the C-reactive protein levels,rs1130864 gene polymorphism and cerebral infarction. Methods A case-control study on 105 patients with cerebral infarction and 121 healthy controls was carried out. The high-sensitive C reactive protein was determination by immune-turhiclimetry, the genotype and allele frequencies of rs1130864 gene polymorphism were assayed by polymerase chain reaction-restricted fragments length polymorphism （ PCR-RFLP）. All the patients were measured with National Institutes of Health Stroke Scale（NIHSS）. Results There were no statistical significant for rs1130864 genotype frequencies between the pa- tients with cerebral infarction and the healthy controls（P 〉0.05 ） , and there were no statistical significant difference that neither CRP genotypes nor alleles showed an association with patient~ condition（P 〉 0.05 ）. The risk factors for cerebral infarction, including history of hypertension, diabetes, smoking, HDL-C, hs-CRP level. Conclusion rs1130864 polymorphism is not associated with cerebral infarction in Chines.