碳酸酐酶Ⅱ在胰腺浸润性导管癌中的表达及其临床意义

Clinicopathological significance of the expression of carbonic anhydrase Ⅱ in human pancreatic invasive ductal cancer

目的探讨胰腺浸润性导管癌（IDC）中碳酸酐酶Ⅱ（CAⅡ）蛋白和mRNA表达水平及其临床病理学意义。方法应用免疫组化方法检测33例胰腺IDC组织及配对的癌旁组织石蜡标本CAⅡ蛋白表达水平，观察其与患者临床病理学参数的关系；再用Westernblot和逆转录聚合酶链式反应RT-PCR检测12例冷冻保存的新鲜配埘IDC组织、癌旁组织中CAⅡ蛋白和mRNA表达水平。结果免疫组化结果显示：CAⅡ在11例胰腺IDC中呈高表达（33．3％），明显低于配对癌旁组织的表达水平（72．7％，t=6．275，P=0．000）。CAⅡ的表达与胰腺IDC的发生部位（X2=0．992，P=0．319）、分化程度（X2=0．866，P=0．352）、TNM分期（X2=1．210，P=0＋271）和淋巴结转移（X2=0，798，P=0．372）无明显相关性，但与患者预后的相关性接近统计学意义（X2=3．233，P=0．072）。高表达CAⅡ的胰腺IDC患者中位生存时间为540d，低表达CAⅡ者为320d。RT—PCR和Westernblot结果显示，CAⅡ在胰腺IDC中的蛋白和mRNA表达均低于配对癌旁组织（t=3．399，P=0．006；t=2．281，P=0．043）。结论CAⅡ在胰腺IDC中表达下调，并可能与患者预后相关。

Objective To study the clinicopathologieal significance of the expression of carbonic anhydrase （CA） Ⅱ protein and mRNA in primary invasive ductal cancer （IDC） of human pancreas. Methods The expression of CA Ⅱ protein in 33 paired paraffin embedded IDC specimens of the pancreas and paired adjacent non-cancerous pancreatic tissues was detected by immunohistochemistry. Western blot and reverse transcription polymerase chain reaction （RT-PCR） were used to examine the expression of CAⅡ protein and mRNA level in 12 paired fresh IDC specimens of the pancreas and adjuvant non-cancerous pancreatic tissues. The relationship between the protein expression and clinicopathological features was analyzed. Results Overexpression of CA Ⅱ protein was shown in 11 cases of pancreatic [DC tissues （33.3% , 11/33 ）, which was much lower than that in paired non-cancerous pancreatic tissues （72. 7% , t = 6. 275, P = 0. 000）. The expression of CAⅡ protein had no correlation with tumor position （ X2 = 0. 992, P =0. 319）, differentiation （X2 =0. 866, P =0. 352）, TNM stage （X2 = 1. 210, P =0. 271 ） and Lymph node metastasis （X2 =0. 798, P =0. 372） , but had bordering statistic sig with the prognosis of the patients （X2 = 3.233, P =0.072）. The median survival time in the patients with high expression of CA Ⅱ protein was 540 days, while that in the patients with low expression was 320 days. The expression of CAⅡ protein and mRNA was lower in IDC than that in paired non-cancerous pancreatic tissues detected by Western blot and RT-PCR respectively （t=3.399, P=0.006; t=2.281, P=0.043）. Conclusion CAⅡ is down regulated in pancreatic IDC and might be relative with the prognosis.