肺炎军团杆菌诱导NCI-H292细胞β-defensin-2表达的分子机制

Molecular mechanism of β-defensin-2 expression in Legionella pneumoniae induced NCI-H292 cells

目的探讨人β防御素2(human beta defensin 2,hBD-2)基因作用的分子机制,为临床抗感染治疗提供依据。方法肺炎军团杆菌感染NCI-H292细胞,采用菌落形成实验,Western blot实验和ELISA实验检测丝裂原活化蛋白激酶(mi-togen-activatived protein kinase,MAPK)途径内基因的变化。结果 hBD-2对肺炎军团杆菌有抑制作用,肺炎军团杆菌诱导分泌hBD-2主要是通过JNK和p38途径起作用。结论肺炎军团杆菌主要通过c-Jun氨基末端激酶(the c-Jun N-termi-nal kinase,JNK)和p38途径诱导NCI-H292细胞分泌hBD-2。

Objective To investigate the role of hBD-2 gene for the clinical anti-infective therapy.Methods Legionella pneumoniae infection in NCI-H292 cells,using colony formation assay,Western blotting,and ELISA to investgate the related gene changes in the MAPK pathway.Results hBD-2 inhibited Legionella pneumoniae,and Legionella pneumoniae induced secretion of hBD-2 mainly through the JNK and p38 pathway.Conclusions Legionella pneumoniae induces hBD-2 secreted from NCI-H292 cells,which is mainly due to JNK and p38 pathway.